Apollo Sci. Cefotaxime sodium salt

Cefotaxime Sodium Salt, Cell Culture Grade is a high-purity, broad-spectrum cephalosporin antibiotic widely used in cell culture applications and microbiological research. As a third-generation cephalosporin, cefotaxime sodium salt is particularly effective against a range of Gram-negative and some Gram-positive bacteria. It is used primarily for selective pressure in cell culture systems and for studying antimicrobial activity in research environments.

Key Features

1. High Purity: Formulated as cell culture grade for reliable use in sensitive biological systems.
2. Broad-Spectrum Activity: Effective against a wide variety of Gram-negative and Gram-positive bacteria, including beta-lactamase-producing strains.
3. Water-Soluble: Easily dissolves in aqueous solutions for convenient application in cell culture and microbiology.
4. Beta-Lactam Antibiotic: Inhibits bacterial cell wall synthesis by binding to penicillin-binding proteins (PBPs), leading to bacterial lysis.
5. Selective Pressure Agent: Used to maintain plasmid selection and prevent contamination in cell culture systems.

Applications

1. Cell Culture:
   • Provides selective pressure to maintain genetically modified cells.
   • Prevents bacterial contamination in mammalian or microbial cell culture systems.
2. Antimicrobial Research:
   • Used in antimicrobial susceptibility testing to study resistance mechanisms.
   • Aids in the evaluation of novel antibacterial agents.
3. Microbiology:
   • Supports the isolation and identification of specific bacterial strains.
   • Used in media formulations for selective bacterial growth inhibition.

Specifications

• Chemical Formula: C₁₆H₁₇N₅O₇S₂
• Molecular Weight: 477.45 g/mol
• Form: White to off-white powder
• Solubility: Highly soluble in water
• Grade: Cell culture grade, suitable for sensitive biological systems

How It Works

Cefotaxime sodium salt inhibits bacterial cell wall synthesis by interfering with the cross-linking of peptidoglycan, a critical component of the bacterial cell wall. It is effective against many beta-lactamase-producing organisms, providing a broad range of antibacterial activity.

Storage and Stability

• Storage Conditions: Store at 2–8°C in a tightly sealed container to maintain stability.
• Stability: Stable under recommended storage conditions; reconstituted solutions should be used promptly or stored at –20°C for short-term use.

Safety and Handling

• Precautions: Handle with care; avoid inhalation, ingestion, and contact with skin or eyes.
• Protective Equipment: Use gloves, lab coat, and safety glasses when handling.
• Disposal: Dispose of in accordance with local biohazard waste regulations.

Product Benefits

• Reliability: High purity ensures consistent performance in cell culture and microbiology applications.
• Versatility: Suitable for both research and industrial applications in microbiology, cell culture, and antimicrobial studies.
• Efficiency: Provides effective and selective bacterial inhibition, ensuring contamination-free experiments.

Apollo Scientific Cefotaxime Sodium Salt, Cell Culture Grade, is a trusted and versatile antibiotic reagent that meets the stringent demands of modern research and industrial microbiology.