Human Immunodeficiency Virus 1+2 Antibody Plus P24 Antigen (Anti-HIV-1/2 + P24-Ag) ELISA Kit

Qualitative ELISA for Simultaneous Detection of HIV-1/2 Antibodies and p24 Antigen in Human Samples

The Human HIV (1+2 Antibodies Plus p24 Antigen) ELISA Kit is a colorimetric, sandwich-based enzyme-linked immunosorbent assay (ELISA) designed for the qualitative detection of antibodies against HIV-1 and HIV-2 (IgG, IgM, IgA) and HIV p24 core antigen in human serum, plasma, tissue homogenates, and other biological fluids.

This combined antibody/antigen detection format improves diagnostic window coverage by enabling detection of early HIV infection (via p24 antigen) as well as established immune response (via HIV antibodies). The assay is highly sensitive and specific, uses a 450 nm optical density readout, and provides a reliable platform for research studies involving HIV exposure, vaccine development, and immunopathology.

⚠ Research Use Only (RUO) – Not for use in diagnostic or therapeutic applications.

✅ Key Features & Benefits

• Dual Detection Capability – Simultaneous detection of HIV-1/2 antibodies and p24 antigen
• Colorimetric, Sandwich ELISA – Enables qualitative measurement via absorbance at 450 nm
• Comprehensive Sample Compatibility – Suitable for serum, plasma, and tissue homogenates
• High Sensitivity & Specificity – Ideal for detecting both early and chronic stages of HIV infection
• Stable & Reliable – Valid for 6 months with <5% activity loss under proper storage
• Ready-to-Use Kit Format – Includes pre-coated plates, standards, detection reagents, substrates, and buffers

🧪 Assay Principle

This is a sandwich ELISA. A monoclonal antibody specific to HIV p24 antigen or antibodies is pre-coated on a 96-well microplate. Samples and standards are added, followed by incubation with biotinylated Detection Reagents A and B. After washing, TMB substrate is added, resulting in a colorimetric change. The intensity of the yellow signal (after adding stop solution) is measured at 450 nm, and results are interpreted by comparing OD values to a predetermined cutoff value.

📦 Kit Contents (For 96 Tests)

🧬 Sample Types

• Serum – Collected using standard serum separator tubes
• Plasma – Collected with EDTA or heparin anticoagulants
• Tissue Homogenates – Prepared in PBS and centrifuged before use

⚠ Avoid hemolyzed or lipemic samples; store samples at −20 °C or −80 °C if not tested immediately

📈 Assay Procedure Overview

1. Add Standards, Controls, and Samples to designated wells
2. Incubate with Detection Reagent A, then Detection Reagent B
3. Wash thoroughly after each step
4. Add TMB substrate, incubate, then add stop solution
5. Read OD at 450 nm within 15 minutes
6. Interpret results based on cutoff calculation from negative control OD + 0.15

⚙ Storage and Stability

🔬 Applications

• HIV Seroconversion Studies
• HIV Vaccine Research and Immunogenicity
• Preclinical Drug Screening
• Virology and Immunology Research
• HIV/AIDS Pathogenesis Studies

⚠️ Important Notes

• This kit is optimized for native samples; detection of recombinant proteins is not guaranteed.
• For accurate quantification, samples must be diluted into the mid-range of the assay.
• This kit is for Research Use Only and not intended for diagnostic use.

Conclusion

The Human HIV (1+2 Antibodies Plus p24 Antigen) ELISA Kit provides a robust and sensitive method for qualitative detection of HIV infection markers. Its dual-detection system enables early and chronic HIV detection within a single assay, making it a valuable research tool for laboratories investigating HIV transmission, vaccine efficacy, seroconversion, and immunopathogenesis. With reliable performance and a complete reagent set, this kit ensures consistent results across a range of biological fluids in research settings.