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    2. Human Immunodeficiency Virus 1+2 Antibody Plus P24 Antigen (Anti-HIV-1/2 + P24-Ag) ELISA Kit

    Human Immunodeficiency Virus 1+2 Antibody Plus P24 Antigen (Anti-HIV-1/2 + P24-Ag) ELISA Kit

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      Specifications:
      Application ELISA-Based Assays
      Storage Temperature 2-8°C
      Product Type Elisa Kit
      Product Brand Abbexa
      Product Grade Molecular Biology

      Human HIV (1+2 antibodies plus p24 antigen) ELISA Kit is an ELISA Kit against HIV (1+2 antibodies plus p24 antigen).


      IntroductionThe human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. HIV infects vital cells in the human immune system such as helper T cells (specifically CD4+ T cells), macrophages, and dendritic cells. HIV infection leads to low levels of CD4+ T cells through a number of mechanisms, including pyroptosis of abortively infected T cells, apoptosis of uninfected bystander cells, direct viral killing of infected cells, and killing of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections.
      TargetImmunodeficiency Virus 1+2 Antibody Plus P24 Antigen (Anti-HIV-1/2 + P24-Ag)
      ReactivityHuman
      Tested ApplicationsELISA
      Recommended dilutionsOptimal dilutions/concentrations should be determined by the end user.
      StorageShipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
      ValidityThe validity for this kit is 6 months.
      StabilityThe stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
      UNSPSC Code41116158
      Standard FormLyophilized
      Detection MethodColorimetric
      Assay TypeSandwich
      Assay DataQualitative
      Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
      Kit ComponentsThe kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
      • Pre-coated 96-Well Microplate
      • Standard
      • Standard Diluent Buffer
      • Wash Buffer
      • Detection Reagent A
      • Detection Reagent B
      • Diluent A
      • Diluent B
      • TMB Substrate
      • Stop Solution
      • Plate Sealer
      Material Required But Not Provided
      • 37°C incubator
      • Multi and single channel pipettes and sterile pipette tips
      • Squirt bottle or automated microplate washer
      • 1.5 ml tubes
      • Distilled water
      • Absorbent filter papers
      • 100 ml and 1 liter graduated cylinders
      • Microplate reader (wavelength: 450 nm)
      • ELISA Shaker
      Sample Collection/Preparation
      • Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4°C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20°C or -80°C.
      • Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 × g within 30 minutes of collection. Assay immediately or aliquot and store at -20°C or -80°C. Avoid hemolysis and high cholesterol samples.
      • Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type – this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 × g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20°C.
      Reagent PreparationThis procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
      • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
      • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
      • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
      Assay ProcedureThis procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
      • 1) Set standard, test samples and control wells.
      • 2) Aliquot 100 µl of diluted standard into the standard wells.
      • 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well.
      • 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
      • 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
      • 6) Wash 3 times.
      • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C.
      • 8) Wash 5 times.
      • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
      • 10) Aliquot 50 µl of Stop Solution.
      • 11) Measure the OD at 450 nm.
      AvailabilityShipped within 5-12 working days.
      NoteThis product is for research use only.

      The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

      Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
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