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Abcam Albumin Creatinine Ratio (ACR) Assay Kit, 100 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | abcam | ||
| Product Grade | Molecular Biology | ||
The Abcam Albumin Creatinine Ratio (ACR) Assay Kit (ab241018) is a sensitive and high-throughput compatible assay designed for the simultaneous quantification of albumin, creatinine, and their ratio (ACR). This kit provides a reliable solution for assessing renal function and early kidney damage, particularly in studies involving microalbuminuria and nephropathy.
The assay combines fluorescence-based detection for albumin and colorimetric detection for creatinine, delivering accurate and reproducible measurements across a wide dynamic range. Its streamlined workflow and complete reagent system make it suitable for both routine and advanced research applications.
Key Features
- Dual detection system for albumin and creatinine in a single kit
- Sensitive measurement of Albumin-to-Creatinine Ratio (ACR)
- Fluorometric detection for albumin (Ex/Em: 600/630 nm)
- Colorimetric detection for creatinine (570 nm)
- Broad detection ranges for both analytes
- High-throughput compatible for 96-well plate formats
- Complete kit with all required reagents included
- Suitable for renal biomarker and metabolic studies
Applications
- Kidney function and renal health assessment
- Microalbuminuria detection and early nephropathy studies
- Diabetes and hypertension research
- Biomarker analysis in urine samples
- Pharmacological and toxicology studies
- Clinical and translational research
Assay Principle
The assay determines ACR in two steps:
-
Albumin Measurement
- Albumin is detected using a specific fluorescent probe (Albumin Blue 580)
- Fluorescence is measured at Ex/Em = 600/630 nm
-
Creatinine Measurement
- Creatinine is enzymatically converted to sarcosine
- Subsequent oxidation produces a chromophore detectable at 570 nm
The Albumin-to-Creatinine Ratio (ACR) is calculated from the quantified values, providing a key indicator of renal function.
Performance Characteristics
| Parameter | Details |
|---|---|
| Albumin Detection Range | 0.02 – 2.5 mg/mL |
| Creatinine Detection Range | 0.002 – 0.5 mg/mL |
| Detection Methods | Fluorometric (albumin), Colorimetric (creatinine) |
| Plate Format | 96-well |
| Throughput | High-throughput compatible |
Kit Contents
- Albumin Assay Buffer II
- Albumin Diluent
- Albumin Probe (Albumin Blue 580)
- Creatinine Assay Buffer
- Creatinine Standard
- BSA Standard II
- Creatinase and Creatininase enzymes
- Sarcosine Enzyme Mix
- OxiRed™ Probe
Product Variant
| Catalog Number | Quantity |
|---|---|
| ab241018 | 100 tests |
Storage & Handling
- Shipped under blue ice conditions
- Store at –20°C
- Follow protocol-specific storage instructions for individual components
Biological Significance
- Albumin: Major plasma protein; elevated urinary levels indicate glomerular damage
- Creatinine: Waste metabolite reflecting kidney filtration efficiency
- ACR: A key indicator of kidney health and early-stage renal disease
Elevated ACR levels are strongly associated with diabetes, hypertension, and chronic kidney disease, making this assay critical for early detection and monitoring.
The Abcam Albumin Creatinine Ratio (ACR) Assay Kit provides a robust and efficient solution for simultaneous measurement of albumin, creatinine, and their ratio. Its dual-detection approach, high sensitivity, and ease of use make it an essential tool for laboratories conducting renal function studies, biomarker research, and disease progression analysis.