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Gibco™ Poly-D-Lysine, 100 mL
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Concentration: 0.1 mg/mL
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Poly-D-Lysine (PDL) is a synthetic, animal-origin–free extracellular matrix (ECM) coating reagent used to enhance cell adhesion and growth on culture-treated plastic and glass surfaces.
With a polymer molecular weight of 50,000–150,000 Daltons, this ready-to-use, sterile-filtered solution at 0.1 mg/mL concentration provides optimal substrate preparation for neuronal and other attachment-dependent cell cultures.
When combined with Nunc™ cell cultureware and Gibco™ B-27™ Supplements or the B-27™ Plus Neuronal Culture System, Poly-D-Lysine ensures superior neuron viability, attachment, and morphological development.
Key Features
- Ready-to-use solution: Pre-diluted to 0.1 mg/mL for immediate application.
- Synthetic, Animal-Origin Free: Eliminates risk of adventitious contaminants.
- Validated for Neuronal Culture: Optimized for use with Gibco™ B-27™ and B-27™ Plus supplements.
- Sterile and Consistent: Sterile-filtered for reproducibility in cell culture experiments.
- Broad Compatibility: Suitable for coating plastic and glass surfaces used in mammalian cell culture.
- Storage: Stable for 12 months when stored at 2–8 °C.
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Mammalian cells, including neurons |
| Concentration | 0.1 mg/mL |
| Form | Liquid |
| Purity Grade | Cell culture grade |
| Sterility | Sterile-filtered |
| Molecular Weight | 50,000–150,000 Daltons |
| Origin | Synthetic, animal-origin free |
| Shelf Life | 12 months |
| Storage Conditions | 2–8 °C |
| Shipping Conditions | Wet ice |
| Intended Use | Research Use Only (RUO) |
Applications
- Enhancement of cell adhesion on plastic or glass culture surfaces.
- Neuronal cell culture, including hippocampal and cortical neurons.
- Preparation of substrates for B-27™-based serum-free systems.
- Support for growth of attachment-dependent mammalian cell lines.
- Use with Nunc™ coated plates and glass-bottom dishes for imaging studies.
The Gibco™ Poly-D-Lysine (0.1 mg/mL) is a high-quality, synthetic ECM coating reagent designed to promote optimal cell adhesion and viability in mammalian cell culture.
Validated with the B-27™ Neuronal Culture System, this sterile, ready-to-use formulation ensures reproducible and consistent attachment for neuronal and adherent cell studies, all while remaining animal origin–free and cGMP-manufactured.