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Gibco™ ExpiSf™ Expression System Starter Kit with Erlenmeyer Flasks
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C, –80°C, Store Cells in Liquid Nitrogen untill ready to use | ||
| Product Type | Culture Medium | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Complete baculovirus-insect cell system for high-yield recombinant protein expression in Sf9 cells
The Gibco™ ExpiSf™ Expression System Starter Kit provides a comprehensive, chemically defined solution for high-efficiency recombinant protein production in Sf9 insect cells using the baculovirus expression vector system (BEVS). This kit integrates optimized components that work in concert to deliver up to 3x higher protein yields than conventional insect expression platforms.
The system features a fast and simplified workflow—enabling protein production in as little as 6–10 days—with robust scalability from deep-well plates to multi-liter shake flasks. The starter kit includes all key reagents and consumables, including ExpiSf CD Medium, ExpiSf Enhancer, ExpiFectamine™ Sf, ExpiSf9 Cells, and disposable Erlenmeyer flasks, making it ideal for labs initiating insect cell protein expression projects.
Key Features
- High Yields: Produces up to three times more protein than traditional baculovirus-based systems.
- Chemically Defined & AOF: Free from yeastolate and animal-origin components for enhanced reproducibility.
- Rapid Turnaround: Obtain protein in 6–10 days, significantly reducing production timelines.
- Simplified Workflow: Requires no further virus amplification, starting from high-titer P0 virus.
- Scalable: Compatible with volumes from 24-well plates to >3 L shake flasks.
- Ready-to-Use Kit Format: Includes transfection reagents, bacmid competent cells, expression vectors, and culture vessels.
Applications
- High-throughput recombinant protein expression in insect cells
- Structural biology and vaccine research
- Enzyme production and functional protein assays
- Expression of glycosylated or complex eukaryotic proteins
- Antigen production for diagnostic and therapeutic R&D
Kit Components & Storage
| Component | Storage Conditions |
|---|---|
| ExpiSf CD Medium, 1000 mL | 2°C to 8°C, protect from light |
| ExpiSf Enhancer, 4 × 1 mL | 2°C to 8°C |
| ExpiFectamine™ Sf Transfection Reagent, 1 mL | 2°C to 8°C |
| ExpiSf9 Cells, 2 × 1 vial | Vapor-phase liquid nitrogen |
| Opti-MEM, 500 mL | 2°C to 8°C |
| pFastBac1 Vector, 10 µg | 2°C to 8°C |
| pFastBac1-Gus Control Vector, 4 ng | 2°C to 8°C |
| MAX Efficiency™ DH10Bac™ Cells, 5 × 0.1 mL | –80°C |
| 125 mL Nalgene™ PETG Erlenmeyer Flasks × 24 | Room temperature |
Technical Specifications
| Specification | Details |
|---|---|
| Cell Line | Sf9 (SFM Adapted) |
| Format | Kit with Erlenmeyer Flasks |
| Expression Vector | pFastBac1 and control plasmid |
| Cloning Method | Restriction Enzyme/MCS |
| Protein Tag | Untagged |
| Promoter | Polyhedrin |
| Expression System | Baculovirus |
| Transfection Reagent | ExpiFectamine™ Sf |
| Product Line | ExpiSf™ |
| Shipping Conditions | Room temperature, wet ice, or dry ice (as applicable) |
| Application | Insect cell protein expression |
| Unit Size | 1 Kit |
Ordering Information
| Catalog Number | Format |
|---|---|
| A38841 | Kit with Erlenmeyer Flasks |
The Gibco™ ExpiSf™ Expression System Starter Kit is the ideal entry point for laboratories seeking a robust, fast, and scalable insect cell protein expression platform. With all key components supplied in one convenient package, users can quickly establish and optimize high-yield protein production workflows with confidence and consistency.