Invitrogen™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit, 500 assays

Sensitive fluorescence-based assay for D-glucose and glucose oxidase detection

The Invitrogen™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit offers a highly sensitive and straightforward method for detecting D-glucose or glucose oxidase activity using a fluorescence microplate reader, fluorometer, or spectrophotometer. Utilizing the Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine), this kit couples the oxidation of D-glucose by glucose oxidase with the peroxidase-mediated conversion of Amplex™ Red into resorufin, a bright, stable, red-fluorescent product.

The assay enables detection of D-glucose concentrations as low as 3 μM and glucose oxidase activity as low as 0.05 mU/mL. It is suitable for continuous or endpoint measurements and is designed to minimize background autofluorescence in most biological samples.

Key Features

• High Sensitivity – Detects as little as 3 μM D-glucose or 0.05 mU/mL glucose oxidase
• Bright, Stable Signal – Produces resorufin with excitation/emission maxima ~571/585 nm
• Low Background – Minimal interference from autofluorescence
• Versatile Applications – Suitable for continuous or discontinuous assays
• Broad Compatibility – Works with microplate readers, fluorometers, and spectrophotometers

Applications

• Quantification of D-glucose in biological, food, and fermentation samples
• Measurement of glucose oxidase activity
• Coupled enzyme assays for β-glucosidase, glucocerebrosidase, and other glucosidases
• Metabolism and cell signaling research
• Biochemical pathway analysis involving carbohydrate metabolism

Assay Principle

1. Glucose oxidase reacts with D-glucose to produce D-gluconolactone and H₂O₂
2. Horseradish peroxidase (HRP) uses H₂O₂ to oxidize Amplex™ Red reagent
3. Resorufin is formed, emitting bright red fluorescence (~585 nm) proportional to glucose concentration or enzyme activity

Specifications

The Amplex™ Red Glucose/Glucose Oxidase Assay Kit provides precision, sensitivity, and versatility for glucose detection and enzyme activity measurement in a range of applications. Its robust signal, low background, and compatibility with standard lab equipment make it ideal for metabolic research, biochemical assays, and high-throughput screening.