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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | 2-30°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
ACK (Ammonium-Chloride-Potassium) Lysing Buffer is specifically formulated for the efficient lysis of red blood cells (RBCs) in biological samples containing white blood cells (WBCs). It is commonly used in processing EDTA-treated whole blood, buffy coats, and bone marrow samples, ensuring optimal recovery and preservation of WBCs for downstream analysis.
Key Features
- RBC Lysis Efficiency:
- Designed to lyse red blood cells while preserving white blood cells and other cellular components.
- Wide Sample Applicability:
- Compatible with EDTA-treated whole blood, buffy coats, and bone marrow samples.
- cGMP Manufacturing:
- Manufactured in an FDA-registered, ISO 13485-certified facility to ensure high quality and reliability.
- Sterile-Filtered Solution:
- Provided as a sterile liquid for ready-to-use applications.
- Long Shelf Life:
- Stable for 12 months from the date of manufacture under recommended storage conditions.
Specifications
| Property | Details |
|---|---|
| Chemical Name | Lysing Buffer |
| Product Line | Gibco |
| Quantity | 100 mL |
| Physical Form | Liquid |
| Shelf Life | 12 months |
| Sterility | Sterile-filtered |
| Storage Conditions | 15–30°C, protect from light |
| Shipping Conditions | Ambient |
| Unit Size | 100 mL |
| Compliance | cGMP, ISO 13485 |
| For Use With | EDTA-treated blood, buffy coats, bone marrow |
| Applications | Research Use Only |
Applications
- Red Blood Cell Lysis:
- Removes RBCs from blood samples to isolate white blood cells or other cellular components.
- Sample Preparation:
- Prepares cell suspensions for flow cytometry, immunoassays, and other analytical techniques.
- Bone Marrow Analysis:
- Facilitates clean sample preparation from bone marrow for cell enumeration and studies.
- Buffy Coat Processing:
- Enhances WBC recovery in layered blood fractions.
Advantages
| Feature | Benefit |
|---|---|
| Ready-to-Use Solution | Simplifies workflows and reduces preparation time. |
| High Compatibility | Effective for multiple sample types, including whole blood, buffy coats, and bone marrow. |
| Sterile Formulation | Reduces contamination risks in sensitive assays. |
| cGMP Manufacturing | Ensures consistent quality and reliability. |
| Room Temperature Storage | Convenient storage conditions with no need for refrigeration. |
Storage and Handling
- Storage Temperature: 15–30°C.
- Protect from Light: Store in a location away from direct light exposure.
- Shelf Life: 12 months from the date of manufacture.
- Shipping: Shipped under ambient conditions to ensure stability during transit.
Regulatory Information
- For Research Use Only (RUO): Not for diagnostic or clinical procedures.
- Compliant with cGMP and ISO 13485: Ensures adherence to high manufacturing standards.
ACK Lysing Buffer by Gibco™ is an essential tool for laboratories processing blood and bone marrow samples, offering reliability, efficiency, and ease of use.
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