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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Standardized Compensation Beads for Fluorochrome-Conjugated Mouse Antibodies in Flow Cytometry
The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a simple, consistent, and accurate method for establishing single-color compensation controls when using fluorochrome-conjugated mouse antibodies in multicolor flow cytometry. These 6 μm polystyrene beads are optimized for compatibility with all laser lines (UV to red), making them ideal for diverse cytometry platforms.
The kit includes both positive capture beads, which bind any isotype of mouse antibody, and negative beads, which remain non-fluorescent—creating a bimodal signal necessary for precise compensation setup, even when cellular antigens are dim or variably expressed.
Key Features and Benefits
-
Mouse Isotype Compatibility
Captures all isotypes of mouse IgG and IgM antibodies (IgG1, IgG2a, IgG2b, IgG3, IgM). -
Universal Laser Compatibility
Suitable for all excitation wavelengths, including 405 nm, 488 nm, 561 nm, 633/635 nm, and more. -
Bright and Distinct Compensation Signals
Positive beads provide strong, high-intensity fluorescence, ideal for defining compensation settings in any flow cytometry panel. -
Minimizes Variability from Cell-Based Controls
Overcomes issues with dim or non-uniform cellular staining by using non-biological, reproducible controls. -
Ready-to-Use Format
Contains pre-aliquoted beads in a convenient dropper vial for quick and reproducible setup.
Applications
- Single-color compensation control for multicolor flow cytometry
- Compensation standardization when using fluorophore-conjugated mouse antibodies
- Multicolor panel optimization and validation workflows
- Immune cell subset analysis using murine reagents
- Intracellular and surface staining compensation
Technical Specifications
| Property | Details |
|---|---|
| Product Name | AbC™ Anti-Mouse Bead Kit |
| Catalog Number | A10344 |
| Bead Size | 6 µm |
| Laser Compatibility | All lasers (UV to 633/635 nm) |
| Species Reactivity | Mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) |
| Format | Tube (1 vial capture beads, 1 vial negative beads) |
| No. of Tests | 100 tests |
| Detection Method | Fluorescence |
| Stain Configuration | Surface (for antibody capture) |
| Instrument Compatibility | Flow cytometer |
| Storage Conditions | 2°C to 8°C |
| Shipping Conditions | Room temperature |
| Product Line | CompenFlow™, Molecular Probes™ |
| Unit Size | Each |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Kit Contents
- 1 vial of AbC™ capture beads (positive control)
- 1 vial of negative beads (non-reactive control)
The Invitrogen™ AbC™ Anti-Mouse Bead Kit is an essential reagent for flow cytometry researchers working with mouse antibody panels. With universal laser compatibility, isotype flexibility, and a robust bimodal signal, this kit provides reliable compensation controls for high-dimensional flow cytometry, enabling accurate data interpretation and improved reproducibility across experiments.