Liofilchem® Posaconazole Pos Antimicrobial Susceptibility Disc, 5 µg

Liofilchem® Posaconazole (POS) Antimicrobial Susceptibility Disc (5 µg) is a high-quality, ready-to-use paper disc impregnated with 5 micrograms of posaconazole, a broad-spectrum triazole antifungal agent. Designed for use in the Kirby-Bauer disk diffusion method, this disc enables in vitro antifungal susceptibility testing (AFST) of yeasts and moulds, including clinically relevant Candida and Aspergillus species.

Manufactured under strict ISO 9001 and ISO 13485 quality systems, the Liofilchem® Posaconazole disc ensures reproducible and reliable results in clinical diagnostics, pharmaceutical development, and antifungal resistance surveillance programs.

Key Features

• Accurate dosage: Each disc contains 5 µg of Posaconazole, providing standardized conditions for zone of inhibition measurement.
• Broad-spectrum antifungal coverage: Active against azole-susceptible Candida spp., Aspergillus spp., and some rare moulds.
• Critical for antifungal resistance detection: Particularly relevant for identifying azole resistance in Candida glabrata, Candida auris, and Aspergillus fumigatus.
• CLSI/EUCAST compatible: Suitable for disk diffusion susceptibility testing based on standardized zone interpretation methods.
• Sterile and shelf-stable: Supplied in sealed cartridges or canisters with desiccant to preserve potency.
• Color-coded labeling: Facilitates quick and accurate identification in multi-disc workflows.

Applications and Use Cases

1. Clinical Mycology

• Determine the susceptibility of Candida albicans, C. glabrata, C. krusei, and emerging pathogens like C. auris.
• Assess susceptibility of Aspergillus fumigatus and other filamentous fungi in invasive fungal infections.

2. Antifungal Resistance Surveillance

• Screen for azole-resistant strains in clinical isolates from high-risk patients (e.g., transplant, oncology, ICU).
• Track local and global resistance patterns in healthcare settings.

3. Pharmaceutical and Research Use

• Compare the efficacy of Posaconazole with other azoles such as Itraconazole and Voriconazole.
• Study resistance mechanisms and antifungal pharmacodynamics.

4. Veterinary and Environmental Mycology

• Evaluate antifungal susceptibility in fungal isolates from animals and the environment.

Use Instructions

1. Media: Use RPMI 1640 agar (buffered with MOPS) or other validated fungal susceptibility media.
2. Inoculum Preparation: Standardize fungal suspension to 0.5 McFarland (for yeasts) or appropriate conidial density (for moulds).
3. Inoculation: Swab inoculum evenly over the agar surface using sterile technique.
4. Disc Application: Apply the 5 µg Posaconazole disc using sterile forceps or a disc dispenser.
5. Incubation:
   • Yeasts: 35 ± 2 °C for 24–48 hours
   • Moulds: 35 °C for 48–72 hours
6. Interpretation:
   • Measure zone of inhibition (mm) around the disc.
   • Compare results to EUCAST or CLSI guidelines or internal laboratory standards, where applicable.

Note: Disk diffusion methods for antifungals are less standardized than for antibacterials. Always refer to the most recent EUCAST AFST guidelines or CLSI M44/M61 documents.

Technical Specifications

Quality Control

Each batch is validated using certified fungal strains:

Liofilchem® Posaconazole POS Disc (5 µg) offers a standardized, convenient solution for testing antifungal susceptibility to one of the most important triazole agents in clinical practice. It plays a critical role in managing azole-resistant fungal infections and guiding targeted antifungal therapy. With ISO-certified quality and compatibility with EUCAST/CLSI protocols, it is ideal for clinical, surveillance, and research applications.