Liofilchem® Ketoconazole KCA Antimicrobial Susceptibility Disc, 10 µg

Liofilchem® Ketoconazole KCA Discs (10 µg) are high-quality antifungal susceptibility testing (AFST) discs designed for in vitro use with the Kirby-Bauer disk diffusion method on agar-based media suitable for fungi. Each disc is impregnated with 10 micrograms of Ketoconazole, an imidazole-class antifungal used to evaluate the susceptibility of Candida spp., dermatophytes, and other yeasts and molds. These discs are manufactured under ISO 9001 and ISO 13485 certified quality systems to ensure accurate and reproducible antifungal susceptibility testing in clinical and research laboratories.

Key Features

• Standardized content: Each disc contains 10 µg of Ketoconazole for reliable zone diameter readings.
• Broad-spectrum antifungal: Effective against Candida albicans, Candida tropicalis, Trichophyton spp., and Microsporum spp.
• Validated for clinical and research use: Suitable for disk diffusion susceptibility testing per standardized laboratory practices.
• Sterile and stable: Packaged in sealed cartridges or canisters with desiccant to protect against moisture and contamination.
• Color-coded labeling: Clear disc identification for streamlined use in high-throughput labs.

Applications and Use Cases

1. Clinical Mycology

Used in medical laboratories to:

• Assess susceptibility of:
  • Candida species from oral, vaginal, and systemic infections
  • Trichophyton and Microsporum spp. from skin, nail, and hair samples
• Support antifungal treatment decisions for:
  • Cutaneous candidiasis
  • Dermatophytosis (ringworm, athlete’s foot, onychomycosis)
  • Seborrheic dermatitis and fungal skin conditions

2. Veterinary Mycology

• Test fungal pathogens in animals with skin, ear, or systemic fungal infections.

3. Antifungal Research and Development

• Monitor resistance trends in dermatophytes and yeasts to Ketoconazole.
• Compare efficacy with other azole antifungals (e.g., Fluconazole, Itraconazole).
• Evaluate new formulations or delivery systems for topical or systemic use.

Use Instructions

1. Prepare Mueller-Hinton agar supplemented with 2% glucose and 0.5 µg/mL methylene blue (for yeasts), or Sabouraud Dextrose Agar for dermatophytes.
2. Standardize fungal inoculum to 0.5 McFarland using a turbidity standard or densitometer.
3. Inoculate the plate evenly with a sterile swab.
4. Apply one Ketoconazole disc (10 µg) using sterile forceps or a disc dispenser.
5. Incubate at 28–30 °C for dermatophytes (up to 5–7 days), or at 35 ± 2 °C for yeasts (24–48 hours).
6. Measure the inhibition zone diameter in millimeters.
7. Interpret results using internal lab standards or reference publications (note: CLSI/EUCAST breakpoints for Ketoconazole may be limited or organism-specific).

Storage and Shelf Life

• Storage Conditions: 2–8 °C (refrigerated), sealed with desiccant.
• Shelf Life: 24–36 months from the date of manufacture under appropriate storage conditions.

Liofilchem® Ketoconazole KCA Discs (10 µg) are reliable diagnostic tools for assessing fungal susceptibility to Ketoconazole, especially in dermatological and mucosal infections. With proven performance against dermatophytes and yeasts, these discs support evidence-based antifungal therapy and resistance monitoring. Manufactured to meet international quality standards, they are essential for use in clinical mycology labs, veterinary diagnostics, and antifungal research.