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Thermo Scientific™ Pierce™ Micro-Spin Columns
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Specifications:
| Application | Nucleic Acid Extraction |
| Storage Temperature | Room Temperature |
| Product Type | Sample Prep |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Pierce™ Micro-Spin Columns are designed for handling small volumes of resin and sample, enabling efficient protein purification and sample preparation in a microcentrifuge format. These versatile spin columns are ideal for affinity purification techniques, offering precise and reliable performance.
Key Features
- Small Volume Handling:
- Compatible with resin volumes of 5 to 100 µL.
- Total column capacity: 400 µL.
- Efficient Filtration:
- Equipped with polyethylene filters (30 µm pore size) to retain resin while allowing liquid to pass through.
- Ensures minimal loss of resin and sample.
- Convenient Design:
- Includes O-ring screw top caps for secure closure.
- Comes with press-on bottom caps for easy sample manipulation.
- Compatible with Microcentrifuge:
- Facilitates fast and efficient sample processing through centrifugation.
- Durability and Storage:
- Columns are stable at room temperature, offering long-term usability.
Applications
- Affinity Purification:
- Purification of target proteins using affinity resins (e.g., His-tag, GST-tag, antibody-based resins).
- Immunodepletion:
- Removal of specific proteins or components from samples.
- Immunoprecipitation (IP):
- Isolation of antigens or proteins using antibodies bound to resin.
- Co-immunoprecipitation (co-IP):
- Study protein-protein interactions by isolating complexes bound to an antibody.
Specifications
| Attribute | Details |
|---|---|
| Column Volume | 400 µL |
| Resin Volume Range | 5 to 100 µL |
| Filter Type | Polyethylene, ∼30 µm pore size |
| Cap Type | O-ring screw top with press-on bottom cap |
| Capacity (Metric) | 2 mL |
| Format | Microcentrifuge, Spin Column |
| Column Type | Empty Column, Polyethylene Filter |
| Unit Size | 50 columns |
| Storage Conditions | Room temperature |
Benefits
- Ease of Use:
- Simplifies the process of protein purification and sample preparation.
- Enables precise handling of small sample and resin volumes.
- Time Efficiency:
- Compatible with centrifugation for rapid washing and elution.
- Versatility:
- Suitable for a wide range of affinity-based purification techniques.
- Reliability:
- Minimizes resin loss and sample contamination.
Related Products
- Pierce™ Spin Columns - Screw Cap
- Pierce™ Spin Cups - Paper Filter
- Pierce™ Spin Cups - Cellulose Acetate Filter
- Pierce™ Spin Columns - Snap Cap
Contents and Storage
- Includes 50 columns per pack.
- Store at room temperature for long-term stability.
The Thermo Scientific™ Pierce™ Micro-Spin Columns are a valuable tool for researchers requiring efficient and precise protein purification workflows. Their small-volume compatibility and centrifuge-ready design make them indispensable for immunoprecipitation, affinity purification, and related applications.