Thermo Scientific™ 96-Well Plates for Pierce™ Quantitative Fluorometric Peptide Assay (Black)
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Specifications:
Application | Fluorescence-Based Assay |
Material | Polystyrene |
Storage Temperature | Room Temperature |
Product Type | Plate |
Product Brand | Thermo Fisher Scientific™ |
Product Grade | Molecular Biology |
Designed specifically for use with the Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay, these 96-well polystyrene microplates enable high-throughput and sensitive quantitation of peptides and peptide digests. Their low background fluorescence and high optical clarity support accurate detection of amine-labeled peptides using a microplate fluorometer (excitation at 390 nm / emission at 475 nm).
These plates are optimized to support the mix-and-read fluorescence assay format and provide consistent, reproducible results in protein and peptide quantitation workflows, especially those involving mass spectrometry (MS).
Key Features & Benefits
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Fluorescence-Optimized Microplates
Low autofluorescence background and high signal-to-noise ratio for peptides labeled via amine-reactive fluorophores. -
High Sensitivity
Detects peptide concentrations as low as 5 µg/mL with minimal sample volume (10 µL per well). -
High Throughput Format
96-well plate format supports multiple replicates, controls, and standard curves per assay run. -
Consistent Quality
Plates are tested for compatibility with fluorescent peptide detection at 390/475 nm and provide lot-to-lot reproducibility. -
Convenient Packaging
Supplied as 25 individually wrapped 96-well plates for long-term usability and reduced contamination risk.
Recommended Applications
- Quantitative peptide measurement for proteomics workflows
- Sample normalization prior to LC-MS/MS
- Monitoring peptide recovery after cleanup procedures
- Routine peptide concentration validation in synthetic or digested samples
- Assay development for peptide analysis in biomarker research
Technical Specifications
Attribute | Details |
---|---|
Product Type | 96-Well Fluorescent Microplates |
Plate Format | Standard ANSI/SLAS 96-well layout |
Plate Material | Optically clear polystyrene |
Detection Method | Fluorescence (Ex/Em 390/475 nm) |
Compatibility | Microplate fluorometers |
Recommended Use | Pierce™ Fluorescent Peptide Assay (Cat. No. 23290) |
Sample Volume | 10 µL per well |
Unit Size | 25 plates |
Packaging | Individual plate wrap |
Storage Conditions | Room temperature |
The Thermo Scientific™ 96-Well Plates for Pierce™ Quantitative Fluorescent Peptide Assay provide a dedicated, high-performance platform for accurate, high-throughput peptide quantification. When paired with the Pierce™ Fluorescent Peptide Assay Kit, they ensure consistent, low-background fluorescence and optimized assay performance, particularly in LC-MS sample prep and proteomics studies.