Thermo Scientific™ DMEM for Pierce™ Primary Cell Isolation Kits

Thermo Scientific™ Pierce™ DMEM for Primary Cell Isolation is a high-glucose Dulbecco’s Modified Eagle Medium (DMEM) optimized for isolating and culturing primary cells, particularly neonatal mouse and rat cardiomyocytes. This specialized medium is designed to enhance cell viability, yield, and functionality, making it ideal for primary cell culture, differentiation, and functional assays.

Unlike conventional DMEM formulations, this version has been validated for enzymatic tissue digestion and cardiomyocyte enrichment, ensuring optimal growth and survival of isolated cardiac cells. The media works in conjunction with Pierce™ Primary Cardiomyocyte Isolation Kit for efficient and reproducible cell isolation.

Key Features & Benefits

1. Optimized for Primary Cardiomyocyte Isolation & Culture
   • Enhances cardiomyocyte viability, differentiation, and contractile function.
   • Works seamlessly with enzymatic digestion protocols for high-purity isolation.
2. High-Glucose DMEM for Enhanced Cell Metabolism
   • Supports rapid proliferation and metabolic demands of primary cardiomyocytes.
   • Provides energy-rich conditions for long-term culture and differentiation.
3. Pre-Mixed, Ready-to-Use Formulation
   • Eliminates the need for additional media preparation.
   • Ensures consistent results across multiple experiments.
4. Reduces Cell Stress & Improves Yield
   • Provides up to 4x higher yield compared to traditional isolation methods.
   • Maintains higher cell viability than DIY and other commercial kits.
5. Validated for Preclinical Drug Discovery & Predictive Disease Models
   • Isolated cardiomyocytes express validated biochemical markers and exhibit functional beating activity.
   • Ideal for ischemia, hypoxia, and cardiotoxicity studies.

Product Specifications

Use Cases & Applications

1. Primary Cardiomyocyte Isolation & Culture

• Enables High-Yield & High-Viability Cell Isolation
  • Used for neonatal mouse and rat heart cell dissociation and culture.
  • Maintains cardiomyocyte contractile function for up to 7 days.
• Supports Long-Term Cardiomyocyte Growth & Maintenance
  • Optimized to enhance beating activity and metabolic function.
  • Reduces fibroblast contamination, improving cardiomyocyte purity.

2. Immunohistochemistry (IHC) & Functional Assays

• Supports Cardiac Protein Marker Expression Analysis
  • Suitable for fluorescence-based immunostaining and microscopy.
  • Enhances cardiomyocyte survival for extended IHC workflows.
• Electrophysiological & Contraction Studies
  • Enables detailed analysis of cardiomyocyte contraction and calcium signaling.
  • Ideal for preclinical research in cardiovascular diseases.

3. Preclinical Drug Discovery & Toxicology Studies

• Cardiotoxicity Testing for Drug Candidates
  • Allows screening of pharmacological agents affecting cardiomyocyte function.
  • Supports high-throughput testing for drug-induced cardiac effects.
• Disease Modeling for Cardiovascular Research
  • Provides an in vitro model for ischemia, hypoxia, and hypertrophy studies.
  • Enables target validation and therapeutic screening.

4. Cardiovascular Regenerative Medicine & Tissue Engineering

• Facilitates Cardiomyocyte Differentiation from Stem Cells
  • Used for induced pluripotent stem cell (iPSC) differentiation into cardiomyocytes.
  • Supports tissue engineering applications in heart regeneration.
• Growth Factor Studies & Cytokine Signaling Research
  • Evaluates the role of VEGF, TGF-β, and Wnt signaling in cardiomyocyte development.
  • Helps identify potential regenerative medicine targets.

Instructions for Use

Cell Isolation & Culture Protocol:

1. Tissue Preparation & Digestion:
   • Wash neonatal heart tissue with HBSS (without Ca²⁺, Mg²⁺).
   • Digest with Cardiomyocyte Isolation Enzyme 1 & 2.
2. Cell Washing & Collection:
   • Centrifuge and resuspend isolated cells in HBSS (without Ca²⁺, Mg²⁺).
   • Wash cells twice to remove enzyme residues.
3. Cell Culture & Maintenance:
   • Resuspend in DMEM for Primary Cell Isolation supplemented with Cardiomyocyte Growth Supplement.
   • Plate cells in pre-coated culture plates for differentiation and functional studies.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 4°C, protected from light.
  • Do not freeze, as freezing may alter media composition.
• Handling Precautions:
  • Use aseptic techniques when handling media.
  • Work in a biosafety cabinet (BSC) to prevent contamination.
• Disposal Guidelines:
  • Dispose of used media according to institutional biosafety regulations.
  • If used with biohazardous materials, autoclave before disposal.

Why Choose Thermo Scientific™ DMEM for Pierce™ Primary Cell Isolation Kits?

✅ Optimized for cardiomyocyte isolation & culture, ensuring high viability & yield

✅ Provides energy-rich, high-glucose formulation for sustained cell growth

✅ Pre-mixed, sterile, ready-to-use media eliminates preparation steps

✅ Supports electrophysiological & contractility assays for cardiac research

✅ Reliable for preclinical drug discovery, toxicology studies, and disease modeling

Thermo Scientific™ DMEM for Primary Cell Isolation Kits is a critical media component for high-yield, high-viability cardiomyocyte isolation and culture, ensuring consistent and reproducible results for cardiac research applications.