Liofilchem® Microbial Identification System Reagent kit for up to 200 Tests

The Identification System Reagent by Liofilchem® is a comprehensive kit designed to provide essential reagents for the accurate and efficient identification of bacteria. This kit is specifically tailored for use with systems such as Enterosystem 18R (ref. 71618), Streptosystem 12R (ref. 72560), and Listeria System 18R (ref. 71640). It ensures proper reagent provision for various biochemical and enzymatic tests that aid in identifying bacterial species.

Kit Contents

• Alpha Naphtol (10 mL vial): Used in the Voges-Proskauer test.
• NaOH 40% (10 mL vial): Also used in the Voges-Proskauer test.
• Hydrogen Peroxide (5 mL vial): For the catalase test.
• Methyl Red (10 mL vial): For the methyl red test.
• Sulphanilic Acid (10 mL vial): For the nitrate test.
• Alpha Naphthylamine (10 mL vial): Complements the nitrate test.
• Kovac’s Reagent (10 mL vial): Used in the indole test.
• PYR Reagent (10 mL vial): For the peptidase test.
• Ninhydrin (10 mL vial): Used in the hippurate test.
• Vaseline Oil (10 mL vial): Maintains anaerobic conditions for certain tests.
• Instruction Sheet: Provides guidelines for reagent use and test interpretation.

Reagent Applications and Principles

1. Voges-Proskauer Test:
   • Reagents: Alpha Naphtol and NaOH 40%.
   • Detects acetoin production via red color change.
2. Catalase Test:
   • Reagent: Hydrogen Peroxide.
   • Detects catalase activity by observing bubble formation.
3. Methyl Red Test:
   • Reagent: Methyl Red.
   • Identifies mixed acid fermentation by red color change.
4. Nitrate Test:
   • Reagents: Sulphanilic Acid and Alpha Naphthylamine.
   • Determines nitrate reduction; red color indicates positive results.
5. Indole Test:
   • Reagent: Kovac’s Reagent.
   • Confirms indole production with a pink-red ring formation.
6. Peptidase Test:
   • Reagent: PYR Reagent.
   • Identifies specific bacterial enzymes by red color change.
7. Hippurate Test:
   • Reagent: Ninhydrin.
   • Detects hippurate hydrolysis via color development.
8. Anaerobic Conditions:
   • Reagent: Vaseline Oil.
   • Ensures an anaerobic environment for decarboxylation tests.

Reagent Specifications

• Alpha Naphtol: 6% (w/v) in ethyl alcohol.
• NaOH 40%: Aqueous sodium hydroxide solution.
• Hydrogen Peroxide: 7.5% (v/v).
• Methyl Red: 0.02% (w/v) in aqueous alcohol solution.
• Sulphanilic Acid: 0.8% (w/v) in 5N acetic acid.
• Alpha Naphthylamine: 0.5% (w/v) in 5N acetic acid.
• Kovac’s Reagent: 5% (w/v) p-dimethylaminobenzaldehyde in hydrochloric acid and isobutyl alcohol.
• PYR Reagent: 0.2% (w/v) in aqueous hydrochloric acid solution.
• Ninhydrin: 7% (w/v) in methoxyethanol.
• Vaseline Oil: Pure liquid paraffin.

Usage and Interpretation

• Refer to the specific package insert for the system in use (e.g., Enterosystem 18R, Streptosystem 12R).
• Perform quality control checks with positive and negative controls for each reagent.
• Interpret results using system-specific instructions to ensure reliability and accuracy.

Quality Control

• Validated with ATCC reference strains:
  • Escherichia coli ATCC® 25922
  • Proteus mirabilis ATCC® 25933
  • Listeria monocytogenes ATCC® 19115
• Regular quality checks ensure batch reliability.

Precautions

• Reagents are classified as hazardous. Consult the Safety Data Sheet (SDS) for safe handling instructions.
• Intended for in vitro diagnostic use only and must be handled by trained personnel under approved laboratory conditions.

Storage and Stability

• Store at 2-8°C away from light.
• Stable for up to 4 days at 18-25°C or 48 hours at 35-39°C during transport.
• Discard if signs of deterioration appear.

Presentation

• Ref.: 80260
• Capacity: 100-200 tests
• Comprehensive reagent range supports diverse biochemical tests.
• Compatible with multiple Liofilchem® systems and competitive diagnostic platforms.
• Ensures accurate bacterial identification through reliable reagent quality.
• Packaged for convenience with clear usage instructions and extended stability under varying conditions.

Applications

• Clinical microbiology labs.
• Environmental and industrial testing.
• Food safety pathogen identification.
• Research and academic microbiology.

This comprehensive reagent kit ensures precise and efficient bacterial identification when used with compatible Liofilchem® diagnostic systems.