Liofilchem® Tergitol 0.15% supplement, 4 x 40mL

The Liofilchem TERGITOL 1.5% Supplement is a sterile liquid additive used in conjunction with Tergitol Agar Base (ref. 610184 / 620184). When incorporated into the agar base, this supplement creates a highly selective medium for the detection, enumeration, and identification of Escherichia coli and coliform bacteria in water samples. Its inhibitory properties, combined with Tergitol NP-7, promote the recovery of coliforms by suppressing Gram-positive bacteria, spore-forming Gram-negative bacteria, and Proteus spp. swarming.

Key Features

• Enhanced Selectivity: Tergitol NP-7 suppresses non-target microorganisms while allowing superior recovery of coliform bacteria.
• Convenient Format: Supplied in ready-to-use liquid bottles for aseptic addition to agar base.
• Compatibility: Specifically designed for use with Tergitol Agar Base, requiring no additional processing or sterilization after addition.
• Validated Performance: Stringent quality control ensures consistent results across various applications.

Composition

• Tergitol NP-7: 15 g
• Deionized Water: 1000 mL

Applications

1. Water Quality Testing:
   • Essential for detecting and quantifying coliforms and E. coli in drinking water, recreational water, and wastewater.
   • Adheres to ISO 9308-1 standards for water microbiology.
2. Food and Beverage Microbiology:
   • Ensures compliance with food safety standards by isolating coliforms in production and finished goods.
3. Environmental Monitoring:
   • Used for microbial contamination assessments in various environmental samples.
4. Research Laboratories:
   • Ideal for studies requiring selective coliform isolation for microbiological research and method validation.

Preparation & Usage Instructions

1. Preparation:
   • Aseptically add 7 mL of TERGITOL 1.5% Supplement to 1 litre of autoclaved and cooled (45-50°C) Tergitol Agar Base.
   • Mix thoroughly to ensure even distribution of the supplement.
2. Additional Supplementation:
   • Add 2.5 mL of TTC 1% Supplement (ref. 80300 / 80430) to 1 litre of base medium after sterilization.
3. Dispensing:
   • Pour the prepared medium into sterile Petri dishes or final containers.
4. Inoculation:
   • Use membrane filtration or direct plating techniques to inoculate the medium.
5. Incubation:
   • For total coliforms: Incubate at 37°C for 24 hours.
   • For E. coli: Incubate at 44°C for 16-24 hours.

Interpreting Results

• Typical Colony Morphology:
  • Escherichia coli: Yellow-orange colonies on a yellow medium.
  • Enterobacter/Klebsiella: Yellow colonies on a yellow medium.
  • Proteus/Salmonella/Shigella: Dark red colonies on a green medium.
• Use further confirmatory tests, if necessary, for precise identification.

Quality Control

1. Appearance Control:
   • Clear to slightly opalescent, colorless, and viscous liquid.
2. Microbiological Testing:
   • Prepared medium is inoculated with the following strains and incubated at 36 ± 2°C for 21 ± 3 hours:

Storage & Shelf Life

• Storage:
  • Store at 2-8°C in its original packaging.
  • Keep away from heat and avoid temperature fluctuations.
• Shelf Life:
  • Valid for 2 years under recommended storage conditions.
  • Discard if any signs of deterioration or contamination are observed.

Packaging

• TERGITOL 1.5% Supplement:
  • Product Code: 80042
  • Packaging: 4 bottles x 40 mL

Safety Information

• Intended Use: For professional use only. Laboratory personnel must be trained in microbiological techniques.
• Precautions:
  • Always follow aseptic handling procedures.
  • Consult the Safety Data Sheet (SDS) for guidance on hazards and safe handling practices.

References

1. Chapman, G.H. (1951). A culture medium for detecting and confirming Escherichia coli in ten hours. American Journal of Public Health, 41:1381.
2. Chapman, G.H. (1947). A superior culture medium for the enumeration and differentiation of coliforms. Journal of Bacteriology, 53:504.
3. Kulp, W., Mascoli, C., Tavshanjian, O. (1953). Use of Tergitol-7 Triphenyl Tetrazolium Chloride Agar in routine sanitary water analysis. American Journal of Public Health, 43:1111.

Product Benefits

• Ensures high specificity and selectivity for coliform enumeration.
• Complies with microbiological standards (ISO 9308-1).
• Simplifies preparation with pre-validated supplementation steps.
• Ideal for diverse applications in water testing, food microbiology, and environmental assessments.

This product is a reliable solution for precise and efficient coliform detection, enabling compliance with regulatory and research requirements.