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Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile, 100 Units
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Specifications:
| Application | PCR, Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent, Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile (UDG/UNG), is a recombinant enzyme derived from Gadus morhua (cod liver). It excises uracil residues from DNA by cleaving the N-glycosidic bond between the uracil base and sugar backbone, generating apyrimidinic (abasic) sites that block replication and hybridization.
Unlike standard UNG enzymes, this heat-labile variant is completely and irreversibly inactivated after 10 minutes at 50 °C, making it ideal for workflows that require carry-over contamination prevention with subsequent PCR, cloning, or sequencing.
Key Features
- Heat-Labile – Fully inactivated after 10 min at 50 °C; no risk of residual activity.
- Specificity – Excises uracil from both single- and double-stranded DNA; inactive on RNA and dT-containing DNA.
- Contamination Prevention – Eliminates uracil-containing DNA from prior PCR amplifications.
- Enhanced Workflow Efficiency – Improves cloning success and site-directed mutagenesis efficiency.
- Purity Guaranteed – Chromatographically purified and tested free from contaminating endonucleases and exonucleases.
- Flexible Applications – Compatible with DNA repair, protein–DNA interaction studies, mutagenesis, and SNP detection.
Applications
- PCR carry-over contamination prevention
- Study of DNA repair and mutation detection
- Increase cloning efficiency of PCR products with dU-primers
- Site-directed mutagenesis enhancement
- Protein–DNA interaction research
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Uracil-DNA Glycosylase (heat-labile) |
| Catalog Number | 78310100UN |
| Quantity | 100 U |
| Concentration | 1 U/µL |
| Source | Recombinant Gadus morhua (cod liver) |
| Activity Definition | 1 U liberates 1 nmol uracil from dU-containing DNA in 1 h at 37 °C |
| Substrates | Single- and double-stranded DNA with uracil |
| Not a Substrate | RNA and dT-containing DNA |
| Inactivation | Complete, irreversible after 10 min at 50 °C |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol |
| Assay Conditions | 70 mM Tris-HCl pH 8.0, 10 mM NaCl, 1 mM EDTA, 100 µg/mL BSA, ³H-dUTP-labeled DNA |
| Storage Conditions | –20 °C |
| Shipping Condition | Dry ice |
| Unit Size | Each |
Ordering Information
| Catalog Number | Quantity | Unit Size |
|---|---|---|
| 78310100UN | 100 U | Each |
The Thermo Scientific™ Heat-Labile Uracil-DNA Glycosylase is an advanced enzyme designed for PCR contamination prevention, cloning, and mutation studies. With its heat-labile properties, it can be irreversibly inactivated after PCR setup, ensuring no interference with downstream amplification or sequencing workflows. Its purity, reliability, and flexibility make it a trusted choice for molecular biology and genetic research laboratories.