Thermo Scientific™ Imject™ Freund's Complete Adjuvant (FCA), 5 x 10mL

Thermo Scientific Imject™ Freund's Complete Adjuvant (FCA) is a widely used nonspecific immune response stimulator designed to enhance antibody production in immunization protocols. FCA is a water-in-oil emulsion containing killed Mycobacterium, which helps to increase the immunogenicity of an antigen when mixed and injected into an animal model.

This product is supplied in 5 x 10 mL ampules, making it convenient for multiple uses while ensuring long shelf life.

Key Features & Benefits

✔ Also known as Complete Freund's Adjuvant (CFA)

✔ Water-in-oil emulsion with heat-killed Mycobacterium tuberculosis

✔ Enhances immune response by promoting a strong and prolonged antigen presentation

✔ Optimized for primary immunization to increase antibody titers

✔ Long shelf life with ready-to-use aliquots

✔ Ideal for research applications in antibody development and vaccine studies

Mechanism of Action

Freund’s Complete Adjuvant (FCA) works by:

1. Depot Formation – The water-in-oil emulsion prolongs antigen retention, allowing for sustained antigen release and long-term stimulation of the immune system.
2. Macrophage Activation – The killed Mycobacterium in FCA stimulates antigen-presenting cells (APCs), enhancing T-cell and B-cell responses.
3. Cytokine Secretion – FCA induces the production of pro-inflammatory cytokines, which help recruit immune cells to the injection site.

Applications

1. Antibody Production & Immunization Studies

• Enhances the immune response when generating polyclonal antibodies in rabbits, mice, rats, guinea pigs, and other animals.
• Improves B-cell activation and T-cell response for higher antibody titers.
• Ideal for boosting immunogenicity of weak or low-molecular-weight antigens.

2. Vaccine Development

• Used in preclinical research to test novel vaccines.
• Helps assess immune response strength in experimental vaccine models.

3. Immunological Research

• Used in studies on autoimmunity, infectious diseases, and cancer immunotherapy.
• Helps mimic chronic inflammatory conditions in research on immune regulation.

4. Model for Chronic Inflammation

• Can be used to induce chronic inflammation in animal models for studies on inflammatory diseases such as arthritis, multiple sclerosis, and granulomas.

Comparison: FCA vs. FIA

Handling & Safety Considerations

✔ Storage: Store at 4°C (Do not freeze).

✔ Handling Precautions:

• Use in a fume hood to minimize exposure.
• Wear PPE (gloves, lab coat, eye protection) when handling.
• Avoid repeated injections in the same site to reduce tissue damage.
  ✔ Toxicity Risks:
• FCA causes significant inflammation and should not be used in humans.
• Use only in approved research models.

Related Products

1. Imject™ Alum Adjuvant – Aluminum-based adjuvant for milder immune response stimulation.
2. Imject™ Freund's Incomplete Adjuvant (FIA) – Similar formulation without Mycobacterium, used for booster immunizations.
3. Thermo Scientific™ Ampule Breakers – Safety tool for breaking glass ampules to prevent injury.

Pack Sizes & Ordering Information

Thermo Scientific Imject™ Freund’s Complete Adjuvant (FCA) is a highly effective immunological adjuvant that enhances antigen-specific immune responses. Its water-in-oil emulsion with killed Mycobacterium ensures long-lasting antigen presentation, making it ideal for antibody production, vaccine development, and inflammatory disease modeling. However, its potent inflammatory effects require careful handling and should only be used for primary immunizations in appropriate research models.