Thermo Scientific™ Mammalian beta-Galactosidase Assay Kit

Simple, colorimetric β-galactosidase activity measurement for mammalian cell lysates

The Thermo Scientific™ Mammalian β-Galactosidase Assay Kit provides a straightforward, colorimetric method for lysing cultured mammalian cells and quantifying β-galactosidase (β-Gal) activity. The kit is ideal for reporter gene assays and biochemical enzyme analysis in mammalian cells, with compatibility for prepared lysates from other species, including bacteria.

This versatile kit can be used as a stand-alone reagent system or as a complete assay kit including cell lysis and detection solutions. It allows for direct measurement in cultured cells using a single, all-in-one lysis-and-assay reagent, or via lysate preparation with Thermo Scientific™ M-PER Mammalian Protein Extraction Reagent, which eliminates the need for scraping or freeze-thaw cycles.

Key Features

• Flexible Workflow – Use as a one-step lysis/detection reagent or with separate lysate preparation
• Colorimetric Readout – Easily measured at 405 nm in a plate reader or spectrophotometer
• Adaptable Formats – Suitable for 96-well microplates or tube assays
• High Throughput Ready – Supports homogeneous screening assays or secondary measurements after cell lysis
• Multipurpose Lysates – Lysates prepared with M-PER can be used for other protein-based assays

Applications

• lacZ reporter gene expression analysis
• Enzyme kinetics and inhibitor screening for β-galactosidase
• High-throughput screening in drug discovery
• Comparative β-galactosidase activity profiling across cell types
• Dual-reporter assays with luciferase, CAT, or other reporter enzymes

Specifications

The Mammalian β-Galactosidase Assay Kit offers speed, flexibility, and compatibility for β-galactosidase reporter gene detection in mammalian systems. Its simple colorimetric readout, adaptable format, and efficient lysis system make it a preferred choice for high-throughput and routine β-galactosidase assays.