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Sigma-Aldrich Nocive Brewers Bacteria Agar Base, modified, suitable for microbiology, NutriSelect® Plus
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Specifications:
| Application | Industrial Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Microbiology grade | ||
Nocive Brewers Bacteria Agar Base from Sigma-Aldrich is a powdered, non-sterile, selective culture medium developed for the detection and enumeration of lactic acid bacteria (LAB) and other bacterial beer spoilers, including Pediococcus, Lactobacillus, Leuconostoc, and Lactococcus species. Part of the NutriSelect® Plus product line, it is intended for use in breweries and beverage quality control laboratories, offering consistent performance at a cost-effective price.
This medium supports microbial testing in beer production, helping detect organisms that could negatively affect flavor, aroma, and shelf stability.
Composition (per liter)
| Component | Concentration |
|---|---|
| Bacteriological agar | 15 g |
| Dextrose | 15 g |
| Maltose | 15 g |
| Yeast extract | 5 g |
| Pancreatic digest of casein | 5 g |
| Beef extract | 2 g |
| Disodium phosphate | 2 g |
| Potassium acetate | 6 g |
| L-cysteine hydrochloride | 0.2 g |
| L-malic acid | 0.5 g |
| Chlorophenol red | 0.07 g |
| Polysorbate 80 | 0.5 g |
Product Details
| Property | Value |
|---|---|
| Product Name | Nocive Brewers Bacteria Agar Base, Modified |
| Synonyms | Pedi-Lacto Selective Beer Agar |
| Product Line | NutriSelect® Plus |
| Form | Powder |
| Quality Level | 200 |
| Sterility | Non-sterile |
| Final pH (at 25 °C) | 5.8 ± 0.2 |
| Shelf Life | Limited (expiry date on label) |
| Pack Size | 500 g |
| Catalog Number | 64198-500G |
| Storage Temperature | Ambient |
| Preparation Requirement | Requires beer and water for rehydration |
| UNSPSC Code | 41171609 |
| NACRES Code | NA.85 |
Suitability
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Selective for:
- Lactobacillus spp.
- Lactococcus spp.
- Pediococcus spp.
- Leuconostoc spp.
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Applications:
- Detection of spoilage organisms in beer
- Bioburden testing in food and beverage production
- Microbiological monitoring in breweries
- Selective enumeration of LAB in fermentation environments
Preparation Instructions
- Suspend 66.3 g of the medium in 500 mL of distilled water and 500 mL of degassed beer.
- Mix thoroughly and heat with frequent agitation until completely dissolved.
- Sterilize in an autoclave at 121 °C for 15 minutes.
- Cool to 50 °C and pour into sterile Petri dishes.
Sigma-Aldrich Nocive Brewers Bacteria Agar Base, Modified is a practical, selective culture medium designed to detect and quantify beer spoilage organisms. With its well-balanced formulation and strong selectivity, it is an effective tool for microbiological quality control in brewing and beverage manufacturing environments.