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Lenti-X™ p24 Rapid Titer Kit (Single Wash)
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Liquid |
| Product Brand | TakaRa Bio | Target Organism Class | HIV-1 Virus |
| Product Grade | Molecular Biology | ||
The Lenti-X p24 Rapid Titer Kit (Single Wash) allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a fast and easy ELISA-based protocol. The test samples are mixed with anti-p24 antibody, HRP-conjugated secondary antibody, and lysis buffer. This mix is then added to the wells of the included microtiter plate (12 x 8-well strips) which are coated with anti-HIV-1 p24 capture antibody and binds the p24 (an HIV-1 core protein) in your test samples. After a single wash step, the specifically-bound p24 in the sample is detected with a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content.
Lenti-X p24 Rapid Titer Kit (Single Wash)
- Fast and easy-to-use p24 ELISA kit to determine the titer of your lentivirus prep (~1.5 hr)
- Broader detection range enables determination of p24 titer with fewer dilution steps and iterations
- Collect lentiviral supernatant, lyse, bind, wash (1X), and detect
- 96-well format in separable 12 x 8-well strips, offering flexibility
- p24 control included
Lenti-X p24 Rapid Titer Kit
- Rapidly determine the titer of your lentivirus prep using a p24 ELISA (~3 hr)
- Collect lentiviral supernatant, lyse, bind, wash (3X), and detect
- 96-well format in separable 12 x 8-well strips offering flexibility
- p24 control included
