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Liofilchem™ Fermentation Broth Base (Dehydrated), 500G
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ISO 11290 Compliant Medium for Carbohydrate Utilization Testing
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Liofilchem | Target Organism Class | Listeria monocytogenes |
| Product Grade | Microbiology grade | ||
The Liofilchem™ Fermentation Broth Base is a high-quality liquid culture medium designed for the differentiation of bacteria based on carbohydrate fermentation. Formulated according to ISO 11290, this medium is particularly recommended for the confirmation of Listeria monocytogenes in food and microbiological testing workflows.
Used in combination with specific carbohydrates (e.g., rhamnose), the medium enables laboratories to assess acid and gas production, supporting reliable identification of bacterial species. The inclusion of bromocresol purple as a pH indicator allows clear visual interpretation of fermentation reactions, making it suitable for routine diagnostic, food safety, and research applications.
Key Features
- Formulated according to ISO 11290 standard for Listeria detection
- Supports carbohydrate fermentation testing for bacterial differentiation
- Ideal for confirmation of Listeria monocytogenes
- Contains bromocresol purple indicator for clear color change (purple → yellow)
- Suitable for acid and gas detection (with Durham tubes)
- High-quality dehydrated medium with 4-year shelf life
- Compatible with food, clinical, and environmental microbiology applications
- Reliable performance from Liofilchem™, a trusted microbiology brand
Applications
- Confirmation of Listeria monocytogenes in food testing
- Carbohydrate utilization and fermentation studies
- Differentiation of bacterial species in pure cultures
- Food microbiology and safety testing
- Clinical microbiology diagnostics
- Environmental microbiological analysis
- Teaching and research laboratories
Principle of the Method
The medium supports bacterial growth and enables detection of carbohydrate fermentation:
- Proteose peptone and meat extract provide essential nutrients for microbial growth
- Sodium chloride maintains osmotic balance
- Bromocresol purple acts as a pH indicator
Interpretation:
-
Positive fermentation (acid production):
Medium changes from purple to yellow -
Gas production:
Observed in Durham tubes -
Negative reaction:
Medium remains purple
This allows clear differentiation of microorganisms based on metabolic activity.
Typical Formula (g/L)
| Component | Concentration |
|---|---|
| Proteose Peptone | 10.0 |
| Meat Extract | 1.0 |
| Sodium Chloride | 5.0 |
| Bromocresol Purple | 0.02 |
| Final pH | 6.8 ± 0.2 at 25°C |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Fermentation Broth Base |
| Brand | Liofilchem™ |
| Format | Dehydrated powder |
| Appearance (Powder) | Light yellow, free-flowing |
| Appearance (Prepared) | Clear to slightly hazy, purple |
| pH | 6.8 ± 0.2 |
| Storage Temperature (Powder) | 10–30°C (dry conditions) |
| Storage (Prepared Medium) | 2–8°C (protected from light) |
| Shelf Life | 4 years |
| Packaging Options | 100 g, 500 g |
| Compliance | ISO 11290 |
| Application | Carbohydrate fermentation testing |
Preparation Method
- Suspend 16 g of powder in 1 L of distilled/deionized water
- Heat to boiling and mix until completely dissolved
- Dispense into tubes with inverted Durham tubes
- Sterilize at 121°C for 15 minutes
- Cool to 45–50°C
- Add carbohydrate solution to a final concentration of 0.5%
- Mix thoroughly before inoculation
Quality Control Performance
| Microorganism | Growth | Colour | Gas |
|---|---|---|---|
| Escherichia coli ATCC® 25922 | Good | Yellow | + |
| Listeria monocytogenes ATCC® 19111 | Good | Yellow | − |
| Staphylococcus aureus ATCC® 25923 | Good | Purple | − |
Advantages
The Liofilchem™ Fermentation Broth Base offers high reliability and flexibility for carbohydrate utilization testing. Its clear colorimetric response ensures easy interpretation, while compliance with ISO 11290 makes it suitable for regulatory and food safety testing.
The ability to customize carbohydrate addition allows laboratories to tailor the medium for specific identification workflows, improving diagnostic accuracy and efficiency.
The Liofilchem™ Fermentation Broth Base (Dehydrated) is a versatile and standards-compliant medium for bacterial differentiation through carbohydrate fermentation. With its robust formulation, clear results, and adaptability, it is an essential tool for laboratories involved in food safety, microbiological diagnostics, and research applications.