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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Liofilchem® Brain Heart Infusion (BHI) Broth is a highly nutritious, general-purpose liquid medium designed for the cultivation of a wide range of aerobic and anaerobic bacteria, including fastidious microorganisms from clinical, food, water, and environmental samples. It is particularly suitable for the plasma coagulase test used to identify coagulase-positive staphylococci, as prescribed by ISO 6888 standards.
The formulation also complies with recommendations from the Clinical and Laboratory Standards Institute (CLSI) for the preparation of inocula in antimicrobial susceptibility testing and from the American Public Health Association (APHA) for microbiological analysis of water and wastewater.
Key Features & Benefits
- ✅ Supports growth of fastidious organisms including Staphylococcus aureus, Streptococcus pneumoniae, Bacteroides fragilis, and Escherichia coli
- ✅ Suitable for plasma coagulase testing (ISO 6888)
- ✅ Compliant with CLSI and APHA methods for microbial testing
- ✅ Available in a variety of formats (tubes, bottles, dehydrated powder)
- ✅ Ideal for clinical, industrial, veterinary, and environmental microbiology
Typical Formula (per liter)
| Component | g/L |
|---|---|
| Enzymatic Digest of Animal Tissues | 10.0 |
| Dehydrated Calf Brain Infusion | 12.5 |
| Dehydrated Beef Heart Infusion | 5.0 |
| Glucose | 2.0 |
| Sodium Chloride | 5.0 |
| Disodium Hydrogen Phosphate (Anhydrous) | 2.5 |
| Final pH at 25 °C | 7.4 ± 0.2 |
Preparation Instructions (Dehydrated Medium)
- Suspend 37 g of BHI powder in 1 L of distilled or deionized water.
- Mix thoroughly and heat to boiling, shaking frequently until completely dissolved.
- Dispense into final containers.
- Autoclave at 121 °C for 15 minutes.
-
(Optional) Reduce media for anaerobic use by:
- Incubating under anaerobic conditions for 18–24 h, or
- Heating to 100 °C in a boiling water bath, with caps loosened during heating and tightened during cooling.
Test Procedures
1. General Cultivation
- Inoculate sample into BHI Broth directly or after plate inoculation.
- Incubate aerobically at 37 ± 1 °C for 24–72 hours.
- Observe for turbidity, which indicates microbial growth.
2. Coagulase Test (ISO 6888)
- Inoculate BHI Broth with a colony from Baird-Parker Agar.
- Incubate at 37 ± 1 °C for 24 ± 2 hours.
- Mix 0.1 mL of culture with 0.3 mL rabbit plasma.
- Incubate at 37 °C for 4–6 hours and assess plasma clotting.
- A clot larger than half the total volume indicates a positive result.
Quality Control
| Test Organism | ATCC / WDCM Code | Growth |
|---|---|---|
| Staphylococcus aureus | WDCM 00034 | Good |
| Escherichia coli | ATCC 25922 | Good |
| Streptococcus pneumoniae | ATCC 6305 | Good |
| Bacteroides fragilis | ATCC 25285 | Good (under anaerobic conditions) |
Incubation conditions:
- Aerobes: 37 ± 1 °C for 24 ± 2 h
- Anaerobes: 40–48 h in anaerobic chamber
Product Presentations
| Format | Packaging | Ref. Code |
|---|---|---|
| Tube | 20 × 2 mL | 24141 |
| Tube | 50 × 5 mL | 27502 |
| Tube | 20 × 9 mL | 24480 |
| Tube | 20 × 10 mL | 24104 |
| Tube | 100 × 10 mL | 26104 |
| Bottle | 6 × 200 mL | 412010 |
| Dehydrated Medium (Powder) | 100 g | 620008 |
| Dehydrated Medium (Powder) | 500 g | 610008 |
| Dehydrated Medium (Powder) | 5 kg | 6100085 |
Appearance
- Dehydrated medium: Free-flowing, homogeneous, light beige powder
- Prepared medium: Clear, amber liquid
Storage and Shelf Life
-
Dehydrated medium: 10–30 °C, dry environment, tightly sealed
- Shelf life: 4 years
-
Prepared medium (bottles): 10–25 °C, away from light
- Shelf life: 3 years
-
Prepared medium (tubes): 10–25 °C
- Shelf life: 2 years
Liofilchem® Brain Heart Infusion Broth is a versatile and robust enrichment medium suitable for the cultivation of a wide variety of microorganisms, including aerobes, anaerobes, and fastidious organisms. Its proven use in coagulase testing (ISO 6888), antibiotic susceptibility testing (CLSI), and water and wastewater analysis (APHA) makes it an essential product for clinical laboratories, pharmaceutical QC, and industrial microbiology applications.