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QIAamp DNA Investigator Kit, for 50 Preps
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Includes 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)
Specifications:
| Application | molecular biology |
| Storage Temperature | Room Temperature |
| Product Type | Molecular Biology Reagent |
| Product Brand | Qiagen |
| Product Grade | Molecular Biology |
The QIAamp DNA Investigator Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.
Features
- Purification of high-quality, ready-to-use DNA manually or on the QIAcube Connect
- Consistent, high yields
- Complete removal of contaminants and inhibitors
Product Details
The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios. Purification of DNA using the QIAamp DNA Investigator Kit can be automated on the QIAcube Connect. The QIAamp DNA Investigator Kit meets ISO 18385 requirements.
Performance
The QIAamp DNA Investigator Kit is suited for DNA purification from trace samples (e.g., from crime scene); genotyping, including fingerprinting and paternity analysis; and routine analysis of reference samples.
After sample lysis, the simple QIAamp DNA Investigator procedure, which is highly suited for simultaneous processing of multiple samples, yields pure DNA in less than 30 minutes. DNA is eluted in Buffer ATE or water, and is immediately ready for use in amplification reactions or for storage at –20°C. The purified DNA is free of proteins, nucleases, and other inhibitors.
Principle
The QIAamp DNA Investigator Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl.
Procedure
Using QIAamp MinElute spin columns for purification of high-quality quality DNA with flexible elution volumes, the QIAamp DNA Investigator procedure consists of 4 steps (see flowchart " Procedure"). The sample is lysed under denaturing conditions with proteinase K. DNA binds to the membrane, contaminants flow through, and residual contaminants are washed away. Pure, concentrated DNA is eluted from the membrane. The QIAamp DNA Investigator Kit is automatable on the QIAcube.
The procedure is designed to ensure that there is no sample-to-sample cross-contamination.
See figures
Applications
The high-quality DNA obtained using the QIAamp DNA Investigator Kit is suitable for direct use in forensic and human identity testing for applications, such as:
- Genotyping, including fingerprinting and paternity analysis
- DNA purification from trace samples (e.g., crime-scene)
- Routine analysis of reference samples
Supporting data and figures
QIAamp DNA Investigator procedure.
Specifications
| Features | Specifications |
|---|---|
| Applications | Real-time PCR, STR analysis |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Sample types | wide range of forensic and human-identity sample materials |
| Processing | Manual |
| Elution volume | 20-100 µl |
| Time per run or per prep | less than 30 minutes |
| Technology | Silica technology |
| Format | Spin column |
| Yield | < 3 µg |