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BD™ CBA Human Th1/Th2 Cytokine Kit II, 80 Tests
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | BD Biosciences | ||
| Product Grade | Molecular Biology | ||
High-Performance Bead-Based Assay for Th1 and Th2 Cytokine Analysis in Human Samples
The BD™ CBA Human Th1/Th2 Cytokine Kit II (Catalog No. 551809) offers a powerful multiplex solution for simultaneously quantifying key Th1 and Th2 cytokines from a single human sample. This kit measures IL-2, IL-4, IL-6, IL-10, TNF, and IFN-γ using a bead-based format optimized for flow cytometry. With optimized reagents and PE detection chemistry, this RUO (Research Use Only) kit supports high-throughput immune profiling for disease studies, therapeutic research, and cytokine response monitoring.
Compatible with EDTA plasma, serum, and cell culture supernatants, the kit delivers robust, reproducible results in just one assay—saving time, reagents, and precious sample volume.
Key Features
Multiplex Assay: Detects 6 cytokines (IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ) simultaneously in one sample
Flow Cytometry-Based Detection: Designed for standard flow cytometers using PE-labeled detection reagents
Wide Sample Compatibility: Validated for serum, EDTA plasma, and tissue culture supernatants
Ready-to-Use Reagents: Includes capture beads, PE detection reagent, standards, controls, buffers, and assay diluent
High Sensitivity and Specificity: Beads with discrete fluorescence intensities enable precise analyte discrimination
Storage and Handling: All components stored at 2–8 °C; do not freeze
Research Use Only (RUO): Intended for non-diagnostic, laboratory-based cytokine analysis
Applications
- Th1/Th2 immune response profiling
- Cytokine monitoring in infectious disease and immunotherapy research
- In vitro stimulation studies in PBMC or whole blood
- Immune cell functional assays
- Translational studies of inflammation and autoimmunity
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | BD™ CBA Human Th1/Th2 Cytokine Kit II |
| Catalog Number | 551809 |
| Detection Method | Flow cytometry using PE detection reagent |
| Analytes | IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ |
| Sample Types | EDTA plasma, serum, tissue culture supernatants |
| Format | Multiplex bead-based assay |
| Number of Samples | Up to 80 samples |
| Reactivity | Human (QC tested) |
| Regulatory Status | RUO – Research Use Only |
| RRID | AB_2868940 |
| Brand | BD™ Cytometric Bead Array (CBA) |
| Storage | 2 to 8 °C (do not freeze) |
Kit Components
| Component | Size / Part Number |
|---|---|
| IL-2 Capture Beads | 0.8 mL – 51-9005736 |
| IL-4 Capture Beads | 0.8 mL – 51-9005715 |
| IL-6 Capture Beads | 0.8 mL – 51-9005738 |
| IL-10 Capture Beads | 0.8 mL – 51-9005740 |
| TNF Capture Beads | 0.8 mL – 51-9005724 |
| IFN-γ Capture Beads | 0.8 mL – 51-9005727 |
| PE Detection Reagent | 4 mL – 51-2541KC |
| Cytokine Standards (lyophilized) | 2 × 0.2 mL – 51-2428KC |
| Cytometer Setup Beads | 1.5 mL – 51-9005731 |
| PE Positive Control Detector | 0.5 mL – 51-9005732 |
| FITC Positive Control Detector | 0.5 mL – 51-9005734 |
| Wash Buffer | 130 mL – 51-2431KC |
| Assay Diluent | 30 mL – 51-2432KC |
| Serum Enhancement Buffer | 10 mL – 51-9002150 |
The BD™ CBA Human Th1/Th2 Cytokine Kit II delivers reliable, high-content cytokine analysis for a range of human immunology research needs. With comprehensive reagent coverage, ease of use, and compatibility with standard flow cytometers, this kit is the ideal choice for researchers studying T-helper cell cytokine dynamics, immune modulation, and inflammatory responses in human samples.