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Millipore™ LB Agar, high salt, suitable for microbiology, NutriSelect® Plus
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Microbiology grade | ||
Millipore™ LB-Agar, High Salt (NutriSelect® Plus) is a high-quality, nutrient-rich solid culture medium designed for the cultivation, maintenance, and propagation of non-fastidious bacteria, particularly Escherichia coli.
This formulation is a high-salt variant of traditional Luria Bertani (LB) Agar, containing an elevated concentration of sodium chloride to support robust bacterial growth and enhanced performance in routine microbiology and molecular biology workflows.
LB-Agar remains one of the most widely used general-purpose media in laboratories worldwide, especially for recombinant DNA work, cloning procedures, plasmid propagation, and microbial culture maintenance.
Key Features and Benefits
High-Salt LB Formulation for Strong Growth
This medium contains 10 g/L sodium chloride, making it ideal for:
- High-density bacterial culture growth
- Standard E. coli propagation
- Molecular cloning and transformation workflows
- Routine microbiological culture applications
The high-salt environment supports vigorous growth and improved colony development.
Nutrient-Rich Composition
LB-Agar, High Salt provides essential nutrients through:
- Casein enzymatic hydrolysate (peptone) – nitrogen and amino acids
- Yeast extract – vitamins and growth factors
- Sodium chloride – osmotic balance and enhanced bacterial growth
- Agar – solidifying agent for plate preparation
Broad Suitability for General Microbiology
This medium is non-selective, supporting the growth of:
- Escherichia coli
- Coliform bacteria
- General non-fastidious bacterial species
It is commonly used in both research and industrial microbiology laboratories.
Ideal for Molecular Biology and Recombinant Strain Maintenance
Luria media are a standard choice for:
- Cultivation of recombinant E. coli strains
- Plasmid amplification
- Genetic manipulation experiments
- Routine microbial strain storage and subculture
LB-Agar, High Salt supports E. coli growth to high optical density (OD600 up to ~7), making it suitable for demanding culture applications.
NutriSelect® Plus Quality Standard
This product is part of the NutriSelect® Plus line, offering:
- Reliable microbiological performance
- Cost-efficient powdered medium format
- Standardized QC for laboratory use
Applications
Millipore™ LB-Agar, High Salt is widely used in:
Microbiological Culture
- Routine cultivation of bacteria
- Colony isolation and enumeration
- General laboratory microbiology
Molecular Biology & Biotechnology
- Maintenance of recombinant E. coli strains
- Cloning and plasmid propagation
- Transformation experiments
- PCR-based phylogenetic studies
Food, Beverage, and Clinical Testing
- General microbial growth support
- Non-selective culture workflows in QC laboratories
Product Specifications
| Parameter | Details |
|---|---|
| Product Name | LB-Agar, High Salt |
| Synonym | Luria Bertani Agar, High Salt |
| Brand | Millipore™ NutriSelect® Plus |
| Format | Dehydrated powder |
| Sterility | Non-sterile |
| Final pH | 7.5 ± 0.2 (25°C) |
| Technique | Microbiological culture |
| Suitability | Non-selective for E. coli, coliforms, general bacteria |
Typical Composition (per Liter)
| Component | Quantity |
|---|---|
| Casein enzymic hydrolysate | 10 g/L |
| Yeast extract | 5 g/L |
| Sodium chloride | 10 g/L |
| Agar | 15 g/L |
Preparation Instructions
- Suspend 40 g of powder in 1 L of purified water
- Heat with frequent agitation and boil for 1 minute
- Sterilize by autoclaving at 121°C for 15 minutes
- Cool to ~45–50°C and dispense into sterile Petri dishes
Ordering Information
| Pack Size | SKU |
|---|---|
| 500 g | 52062-500G-F |
| 2.5 kg | 52062-2.5KG-F |
Unique Advantages
- High-salt LB agar for vigorous bacterial growth
- Standard medium for recombinant E. coli culture
- Excellent for cloning, plasmid work, and genetic studies
- NutriSelect® Plus quality for reliable microbiology performance
- Suitable for research, biopharma, food QC, and clinical labs
Millipore™ LB-Agar, High Salt (NutriSelect® Plus) is a trusted, nutrient-rich general-purpose agar medium widely used for culturing E. coli and other non-fastidious bacteria. Its high-salt formulation makes it especially suitable for molecular biology, recombinant strain maintenance, and routine microbiology workflows.