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Specifications:
| Application | Capillary Electrophoresis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Agilent | ||
| Product Grade | Molecular Biology | ||
The Agilent Bioanalyzer High Sensitivity DNA Analysis Kit provides a reliable and sensitive method for the sizing, quantification, and quality control of double-stranded DNA samples across a range of 50–7000 bp. Designed for use with the Agilent Bioanalyzer system, this assay offers high sensitivity down to 5 pg/µL, making it ideal for low-abundance DNA samples and next-generation sequencing (NGS) library QC.
This kit ensures robust, reproducible results with minimal sample input (1 µL), offering accurate sizing and quantification through internal standards, markers, and ladders. It supports a wide variety of molecular biology applications, from NGS library analysis to PCR amplicon validation.
Key Features
- High Sensitivity – Detect DNA concentrations as low as 5 pg/µL for fragments, or 100 pg/µL for complex DNA (NGS libraries).
- Broad Linear Range – Detects both high and low abundance PCR products.
- Accurate Sizing – DNA sizing up to 7000 bp, normalized to internal and external standards.
- Reliable Quantitation – High reproducibility against precise internal markers.
- Low Sample Requirement – Only 1 µL of sample needed.
- Safe & Efficient – Minimal exposure to hazardous dyes such as ethidium bromide.
- NGS Ready – Optimized for quality control of NGS libraries derived from low-input or few amplification cycles.
Applications
- Quality control of next-generation sequencing (NGS) libraries
- Analysis of PCR amplicons and multiplex PCR products
- Gene expression analysis by RT-PCR
- Restriction fragment analysis
- Detection of targeted cleavage in gene editing studies
- General DNA quality, sizing, and quantification assays
Kit Specifications
| Property | Details |
|---|---|
| Product Type | High Sensitivity DNA Kit |
| Catalog Number | 5067-4626 |
| Compatibility | Agilent Bioanalyzer systems (not compatible with G2938A Bioanalyzer) |
| Detection Range | 5 pg/µL – 100 pg/µL |
| Size Range | 50–7000 bp |
| Sample Volume Required | 1 µL |
| Applications | NGS libraries, PCR amplicons, restriction fragments, gene editing |
| Kit Contents | 10 microfluidic chips, reagents, and consumables |
| Shipping Condition | Standard ambient/cold chain as recommended |
| Safety | For research use only |
Ordering Information
| Catalog Number | Description | Unit Size |
|---|---|---|
| 5067-4626 | Agilent High Sensitivity DNA Kit (10 chips, reagents, consumables) | 1 kit |
The Agilent Bioanalyzer High Sensitivity DNA Analysis Kit offers superior accuracy and sensitivity for DNA fragment analysis, making it indispensable for researchers working with low-input samples, NGS libraries, PCR products, and gene editing studies. With its 5 pg/µL sensitivity, broad size range (50–7000 bp), and streamlined workflow, it ensures reliable QC and reproducibility for advanced molecular biology applications.