Thermo Scientific™ Pierce™ Protein A/G Coated Plate IP Kit is a plate-based immunoprecipitation kit designed to perform IP and co-IP assays in 96-well microplate format without the need for resins, beads, centrifugation, or magnets. The kit uses ready-to-use Protein A/G coated 96-well strip plates to capture antibody-antigen complexes efficiently and reproducibly.
This format is especially useful for laboratories processing multiple samples and controls in parallel. Compared with traditional tube-and-resin immunoprecipitation methods, the plate-based workflow allows faster washing, easier handling, and more convenient processing using standard ELISA tools such as multichannel pipettors, plate washers, and microplate mixers.
Key Features
| Feature | Details |
|---|---|
| Plate-Based IP Workflow | Enables immunoprecipitation in 96-well plates without beads, resin, centrifugation, or magnets |
| Protein A/G Coated Plates | Combines the antibody-binding properties of Protein A and Protein G |
| High Throughput Format | Suitable for processing multiple samples and controls simultaneously |
| Faster Washing | Plate format enables easier and more thorough washing than traditional resin-based methods |
| Strip Plate Convenience | Coated 96-well strip plates allow partial-plate use and reduced reagent waste |
| ELISA Tool Compatibility | Compatible with multichannel pipettors, plate washing workflows, and microplate mixers |
| Complete Kit Format | Includes coated plates, uncoated plates, buffers, detergent solution, and sealing tape |
Applications
Pierce™ Protein A/G Coated Plate IP Kit is suitable for:
- Immunoprecipitation assays
- Co-immunoprecipitation workflows
- Affinity purification of antibody-antigen complexes
- Processing multiple samples and controls in parallel
- Plate-based protein interaction studies
- Antibody-based capture workflows
- Applications where resin-free and bead-free IP is preferred
- Research workflows using microplate-based handling and washing
Product Specifications
| Specification | Details |
|---|---|
| Product Name | Pierce™ Protein A/G Coated Plate IP Kit |
| Catalog Number | 45350 |
| Product Type | Immunoprecipitation / Co-Immunoprecipitation Kit |
| Assay | Affinity Purification |
| Application | Immunoprecipitation |
| Format | Kit |
| Quantity | 2 plate kit |
| Capacity | 2 plates |
| Sufficient For | 192 samples / total IP reactions |
| Target | Not target-specific |
| For Use With Equipment | Microplate mixer |
| Product Line | Pierce™ |
| Unit Size | Each |
| Storage | 4 °C |
| Research Use | For Research Use Only; not for diagnostic procedures |
Kit Contents
| Component | Quantity |
|---|---|
| Protein A/G Coated 96-Well Strip Plates | 2 plates |
| Uncoated 96-Well Strip Plate, White | 2 plates |
| PBS Pack, makes 500 mL | 1 pack |
| Surfact-Amps™ X-100 Detergent Solution | 6 x 10 mL |
| Elution Buffer, pH 2.8 | 50 mL |
| Neutralization Buffer | 12 mL |
| Sealing Tape for 96-Well Plates | 18 sheets |
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 45350 | 2 plate kit |
Storage & Handling
Store kit components at 4 °C. Use appropriate laboratory handling practices and keep plates and reagents protected from contamination. Follow the supplied protocol for sample binding, washing, elution, and neutralization steps.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.
User Guide: Pierce Protein A/G Plate IP Kit
Thermo Scientific™ Pierce™ Protein A/G Coated Plate IP Kit is a plate-based immunoprecipitation kit designed to perform IP and co-IP assays in 96-well microplate format without the need for resins, beads, centrifugation, or magnets. The kit uses ready-to-use Protein A/G coated 96-well strip plates to capture antibody-antigen complexes efficiently and reproducibly.
This format is especially useful for laboratories processing multiple samples and controls in parallel. Compared with traditional tube-and-resin immunoprecipitation methods, the plate-based workflow allows faster washing, easier handling, and more convenient processing using standard ELISA tools such as multichannel pipettors, plate washers, and microplate mixers.
Key Features
| Feature | Details |
|---|---|
| Plate-Based IP Workflow | Enables immunoprecipitation in 96-well plates without beads, resin, centrifugation, or magnets |
| Protein A/G Coated Plates | Combines the antibody-binding properties of Protein A and Protein G |
| High Throughput Format | Suitable for processing multiple samples and controls simultaneously |
| Faster Washing | Plate format enables easier and more thorough washing than traditional resin-based methods |
| Strip Plate Convenience | Coated 96-well strip plates allow partial-plate use and reduced reagent waste |
| ELISA Tool Compatibility | Compatible with multichannel pipettors, plate washing workflows, and microplate mixers |
| Complete Kit Format | Includes coated plates, uncoated plates, buffers, detergent solution, and sealing tape |
Applications
Pierce™ Protein A/G Coated Plate IP Kit is suitable for:
- Immunoprecipitation assays
- Co-immunoprecipitation workflows
- Affinity purification of antibody-antigen complexes
- Processing multiple samples and controls in parallel
- Plate-based protein interaction studies
- Antibody-based capture workflows
- Applications where resin-free and bead-free IP is preferred
- Research workflows using microplate-based handling and washing
Product Specifications
| Specification | Details |
|---|---|
| Product Name | Pierce™ Protein A/G Coated Plate IP Kit |
| Catalog Number | 45350 |
| Product Type | Immunoprecipitation / Co-Immunoprecipitation Kit |
| Assay | Affinity Purification |
| Application | Immunoprecipitation |
| Format | Kit |
| Quantity | 2 plate kit |
| Capacity | 2 plates |
| Sufficient For | 192 samples / total IP reactions |
| Target | Not target-specific |
| For Use With Equipment | Microplate mixer |
| Product Line | Pierce™ |
| Unit Size | Each |
| Storage | 4 °C |
| Research Use | For Research Use Only; not for diagnostic procedures |
Kit Contents
| Component | Quantity |
|---|---|
| Protein A/G Coated 96-Well Strip Plates | 2 plates |
| Uncoated 96-Well Strip Plate, White | 2 plates |
| PBS Pack, makes 500 mL | 1 pack |
| Surfact-Amps™ X-100 Detergent Solution | 6 x 10 mL |
| Elution Buffer, pH 2.8 | 50 mL |
| Neutralization Buffer | 12 mL |
| Sealing Tape for 96-Well Plates | 18 sheets |
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 45350 | 2 plate kit |
Storage & Handling
Store kit components at 4 °C. Use appropriate laboratory handling practices and keep plates and reagents protected from contamination. Follow the supplied protocol for sample binding, washing, elution, and neutralization steps.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.