Invitrogen™ ERCC ExFold RNA Spike-In Mixes Kit

The Invitrogen™ ERCC ExFold RNA Spike-In Mixes are a set of standardized external RNA controls designed to enable precise assessment of gene expression experiments, including RNA sequencing (RNA-Seq), microarray analysis, and PCR-based workflows. Developed by the External RNA Controls Consortium (ERCC) and traceable to NIST-certified reference materials, these spike-in mixes provide a reliable framework for evaluating analytical performance, quantifying differential gene expression, and ensuring reproducibility across experiments and platforms.

This kit contains two distinct spike-in formulations—ExFold Spike-In Mix 1 and ExFold Spike-In Mix 2—each comprising a defined blend of 92 synthetic RNA transcripts. These transcripts range from 250 to 2,000 nucleotides in length and mimic natural polyadenylated eukaryotic mRNAs. By adding different spike-in mixes to separate RNA samples, researchers can directly compare expression fold-change ratios and validate experimental sensitivity, dynamic range, and detection limits with high confidence.

The ERCC ExFold Spike-In Mixes are especially valuable for transcriptomics workflows requiring quantitative comparison across multiple samples, sequencing platforms, or laboratories. Their precisely defined molar concentrations and known expression ratios allow researchers to assess technical variability, normalize expression data, and verify the accuracy of gene expression measurements.

Manufactured under the trusted Ambion™ product line, these spike-in controls provide robust quality assurance for modern genomics and molecular biology applications, including next-generation sequencing, gene expression profiling, and transcriptome analysis.

Key Features and Benefits

• Contains two spike-in mixes for accurate fold-change analysis
• Includes 92 synthetic RNA transcripts traceable to NIST reference materials
• Enables accurate normalization and comparison across gene expression experiments
• Supports evaluation of dynamic range, sensitivity, and detection limits
• Facilitates reliable quantification of differential gene expression
• Mimics natural eukaryotic mRNA transcripts
• Compatible with RNA-Seq, microarrays, and PCR-based assays
• Ensures reproducibility across platforms, samples, and laboratories

Applications

• RNA sequencing (RNA-Seq) normalization and validation
• Differential gene expression analysis
• Transcriptome profiling and quantitative gene expression studies
• Microarray and PCR-based gene expression workflows
• Sequencing platform performance assessment
• Molecular biology and genomics research
• RNA quality control and experimental standardization

Kit Contents

Technical Specifications

Ordering Information

The Invitrogen ERCC ExFold RNA Spike-In Mixes provide a reliable and standardized solution for validating and comparing gene expression data across experiments and platforms. By enabling precise fold-change measurement, sensitivity evaluation, and data normalization, this kit ensures accurate and reproducible transcriptomics results. It is an essential quality control tool for researchers conducting RNA sequencing, gene expression profiling, and molecular biology studies.