Applied Biosystems™ TaqMan™ β-Actin Detection Reagents,100 Rxns

The Applied Biosystems™ TaqMan™ β-Actin Detection Reagents provide a reliable, pre-validated solution for using β-actin (ACTB) as an endogenous normalization control in real-time PCR (qPCR) and RT-qPCR experiments. β-actin is one of the most widely used housekeeping genes due to its stable and consistent expression across a wide range of human tissues and experimental conditions, making it ideal for accurate normalization of gene expression data.

This kit includes pre-designed primers and a FAM-labeled TaqMan™ probe, eliminating the need for assay design and optimization. The probe incorporates a 5′ FAM reporter dye and 3′ TAMRA™ quencher, ensuring highly sensitive and specific fluorescence detection during amplification. The ready-to-use format simplifies workflow setup and improves reproducibility across experiments.

Compatible with both DNA and RNA templates, the TaqMan™ β-actin Detection Reagents can be used in one-step RT-PCR workflows alongside Applied Biosystems™ TaqMan™ master mixes, enabling precise normalization for gene expression analysis, RNA quantification, and molecular biology research applications.

Key Features and Benefits

Pre-Designed and Validated Assay

• Includes optimized β-actin primers and TaqMan™ probe
• Eliminates assay design and optimization time
• Ensures consistent and reproducible results

Accurate Gene Expression Normalization

• Uses β-actin (ACTB), a stable housekeeping gene
• Enables reliable normalization across samples
• Improves quantitative accuracy in gene expression studies

Highly Sensitive and Specific Detection

• FAM reporter dye provides sensitive fluorescence detection
• TAMRA™ quencher reduces background fluorescence
• TaqMan™ probe chemistry ensures superior specificity

Flexible Workflow Compatibility

• Suitable for both DNA and RNA targets
• Compatible with one-step and two-step RT-qPCR workflows
• Works with Applied Biosystems™ real-time PCR systems

Simplified Experimental Setup

• Separate assay components provide flexibility
• Reduces preparation time and experimental complexity
• Ideal for standardized gene expression workflows

Technical Specifications

Compatible Master Mixes

The kit is compatible with:

• TaqMan™ One-Step RT-PCR Master Mix Reagents
• TaqMan™ EZ RT-PCR Core Reagents
• TaqMan™ Gold RT-PCR Reagents
• TaqMan™ Universal PCR Master Mix

Compatible Instruments

Validated for use with Applied Biosystems™ real-time PCR systems, including:

• QuantStudio™ 3, 5, 6 Flex, 7, and 12K Flex
• StepOne™ and StepOnePlus™ Systems
• 7500 and 7500 Fast Real-Time PCR Systems
• ViiA™ 7 Real-Time PCR System
• 7900HT Fast Real-Time PCR System

Applications

The TaqMan™ β-Actin Detection Reagents are ideal for:

• Gene expression normalization
• Relative gene expression analysis
• RT-qPCR control reactions
• RNA expression studies
• Biomarker validation
• Molecular biology research
• Validation of qPCR and NGS data

Workflow Integration

RNA or DNA Template → RT-qPCR Amplification → β-Actin Normalization → Accurate Gene Expression Analysis

The Applied Biosystems™ TaqMan™ β-Actin Detection Reagents provide a convenient, highly sensitive, and validated solution for normalization in real-time PCR and RT-qPCR experiments. With optimized primers, a FAM-labeled probe, and compatibility with Applied Biosystems™ instruments and master mixes, this kit ensures reliable normalization and accurate gene expression quantification in molecular biology and genomics research workflows.