Thermo Scientific™ 1-Step™ ABTS Substrate Solution, 250mL

The 1-Step™ ABTS Substrate Solution is a water-soluble HRP (Horseradish Peroxidase) substrate used in ELISA assays to produce a green end product. This substrate is ideal for applications where lower sensitivity is acceptable or to avoid high background signal caused by more sensitive substrates like OPD or TMB.

Key Features:

1. Water-Soluble Substrate:
   • Produces a green-colored end product upon reaction with HRP.
   • Absorbance peaks at 410 nm and 650 nm.
2. Controlled Sensitivity:
   • Less sensitive than TMB or OPD, reducing background noise in ELISA assays.
   • Slower color development (approximately 20 minutes) provides more control.
3. Ease of Use:
   • Ready-to-use, no additional preparation required.
   • Available in solution or as ABTS Substrate Tablets for added convenience.
4. Non-Toxic Alternative:
   • Provides a safer option for laboratory workflows compared to other substrates.

Specifications:

Applications:

• Quantitative and qualitative ELISA assays.
• Suitable for assays requiring:
  • Controlled sensitivity.
  • Reduced background interference.

Advantages:

1. Reliable Performance:
   • Consistent results with measurable green end products.
2. Reduced Background Noise:
   • Less readily oxidized, ideal for assays prone to high background signals.
3. Longer Reaction Time:
   • Approximately 20 minutes for color development, allowing more control during experiments.
4. Safe and Ready-to-Use:
   • Non-toxic, water-soluble formulation for easy handling.

Comparison with Other Substrates:

Contents & Storage:

• Unit Size: 250 mL.
• Storage: Store at 4°C. Ensure the container is tightly closed to avoid contamination or degradation.

Related Products:

• ABTS Substrate Tablets for flexibility in preparation and storage.

Use Limitations:

• For research use only. Not intended for diagnostic or therapeutic procedures.