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Thermo Scientific™ PMSF Protease Inhibitor, 5g
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Specifications:
| Application | molecular biology, Protein Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Analytical grade | Formula | C₇H₇FO₂S |
Thermo Scientific™ PMSF (Phenylmethylsulfonyl Fluoride) is a widely used serine protease inhibitor designed to protect proteins from enzymatic degradation during cell lysis, protein extraction, and purification workflows. It is particularly effective against key serine proteases such as trypsin, chymotrypsin, thrombin, and papain, making it an essential reagent in protein research laboratories.
Supplied as a high-purity white powder (>99%), PMSF offers reliable performance in biochemical and proteomics applications where targeted inhibition of protease activity is required.
Key Features
Targeted Protease Inhibition
- Inhibits serine proteases including trypsin, chymotrypsin, thrombin, and papain
- Also inhibits certain cysteine proteases (reversible with DTT)
- Suitable for selective protease inhibition strategies
Effective at Low Concentrations
- Recommended working concentration: 0.1–1 mM
- Efficient inhibition in most protein solutions
- Minimizes interference with downstream applications
High Purity and Consistency
- Purity greater than 99%
- Low moisture content (<0.1%)
- Consistent performance across batches
Flexible Preparation
- Easily prepared as stock solutions in organic solvents
- Compatible with ethanol, methanol, or isopropanol
- Suitable for rapid integration into lysis buffers
Applications
- Protein extraction and purification
- Cell lysis workflows
- Enzyme inhibition studies
- Proteomics and protein stability analysis
- Biochemical research and assay development
Mechanism of Action
PMSF irreversibly inhibits serine proteases by reacting with the active site serine residue, thereby blocking enzymatic activity. This prevents proteolytic degradation of proteins during sample preparation.
Additionally, PMSF can inhibit certain cysteine proteases, although this effect is reversible under reducing conditions (e.g., DTT treatment).
Technical Specifications
| Parameter | Specification |
|---|---|
| Chemical Name | Phenylmethylsulfonyl Fluoride (PMSF) |
| Synonyms | α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride |
| Molecular Formula | C₆H₅CH₂SO₂F |
| Molecular Weight | 174.19 g/mol |
| CAS Number | 329-98-6 |
| EC Number | 206-350-2 |
| Purity | >99.0% |
| Appearance | White powder |
| Melting Point | 90–95°C |
| Moisture Content | <0.1% |
| Recommended Concentration | 0.1–1 mM |
Usage Guidelines
- Prepare stock solution at 10 mM (1.74 mg/mL) in isopropanol
- Store stock solution in aliquots at -20°C
- Add to buffer immediately before use due to instability in aqueous solutions
Product Information
| Catalog Number | Description | Quantity |
|---|---|---|
| 36978 | PMSF Protease Inhibitor | 5 g |
Storage and Stability
- Store at 20°C to 25°C in a dry, well-ventilated area
- Protect from light and moisture
- Keep tightly sealed in original container
Related Products
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Thermo Scientific™ PMSF Protease Inhibitor is a reliable and widely used reagent for selective inhibition of serine proteases. With high purity, strong inhibitory activity, and flexible preparation options, it remains an essential component in protein extraction and biochemical research workflows.