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Thermo Scientific™ Phenol/TRIS saturated solution, for molecular biology, stabilized, DNAse, RNAse and Protease free
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Ambient | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₆H₆O |
Molecular biology-grade solvent system for DNA isolation — DNase, RNase, and Protease free
The Thermo Scientific™ Phenol/Tris Saturated Solution is a high-purity, ready-to-use reagent specifically formulated for molecular biology applications, particularly the isolation and purification of DNA. This solution combines phenol and Tris(hydroxymethyl)aminomethane in an aqueous phase, stabilized with hydroxyquinoline to maintain long-term integrity and inhibit oxidation.
Pre-saturated and pH-balanced (pH 7.8–8.2), this reagent eliminates the need for titration and is guaranteed DNase, RNase, and Protease free, ensuring the integrity of nucleic acids during extraction and downstream applications.
Key Features
- Molecular Biology Grade: Free of DNase, RNase, and Protease activity
- Stabilized: Contains 0.08–0.12% hydroxyquinoline for enhanced stability and shelf life
- Ready-to-Use: Pre-saturated and pH-adjusted to 7.8–8.2 (5% in KCl solution)
- Optimized for DNA Isolation: Tris acts as a proton acceptor, raising the pH for effective nucleic acid extraction
- High Purity: Low heavy metal content (≤1 ppm Pb), iron (≤1 ppm Fe), and controlled water content (25–35%)
Applications
- DNA isolation and purification workflows
- Molecular diagnostics and forensic sample preparation
- RNA/DNA co-extraction protocols
- Extraction of nucleic acids from biological specimens
- Research in genomics, molecular biology, and forensic science
Chemical & Technical Specifications
| Property | Value |
|---|---|
| Catalog Number | 327125000 |
| CAS Number | 108-95-2 (Phenol) |
| Chemical Formula | C₆H₆O |
| Molecular Weight | 94.11 g/mol |
| IUPAC Name | Phenol |
| Stabilizer | 0.08–0.12% Hydroxyquinoline |
| Water Content | 25–35% (Karl Fischer) |
| pH | 7.8–8.2 (5% in KCl solution, 25 °C) |
| Iron (Fe) | ≤1 ppm |
| Heavy Metals (as Pb) | ≤1 ppm |
| Enzyme Activity | DNase, RNase, Protease: None detected |
| Appearance (Color) | Yellow to orange-brown |
| Appearance (Form) | Solution |
| Shipping Conditions | Room temperature or wet ice approved |
| Storage Conditions | Store at 2–8 °C; do not freeze |
| Unit Size | 500 mL |
Safety and Handling
Classification of the Substance or Mixture
According to CLP Regulation (EC) No 1272/2008, the product is classified as follows:
| Hazard Class | Category |
|---|---|
| Acute Oral Toxicity | Category 4 |
| Acute Dermal Toxicity | Category 3 |
| Acute Inhalation Toxicity (Vapors) | Category 3 |
| Skin Corrosion/Irritation | Category 1 |
| Serious Eye Damage/Eye Irritation | Category 1 |
| Germ Cell Mutagenicity | Category 2 |
| Specific Target Organ Toxicity – Repeated Exposure (STOT) | Category 2 |
Label Elements
| Signal Word | Danger |
|---|
Hazard Statements (H-phrases):
- H302 – Harmful if swallowed
- H311 + H331 – Toxic in contact with skin or if inhaled
- H314 – Causes severe skin burns and eye damage
- H341 – Suspected of causing genetic defects
- H373 – May cause damage to organs through prolonged or repeated exposure
Precautionary Statements (P-phrases):
- P280 – Wear protective gloves/protective clothing/eye protection/face protection
- P301 + P330 + P331 – IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
- P303 + P361 + P353 – IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water or shower
- P304 + P340 – IF INHALED: Remove person to fresh air and keep comfortable for breathing
- P305 + P351 + P338 – IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P310 – Immediately call a POISON CENTER or doctor/physician
The Thermo Scientific™ Phenol/Tris Saturated Solution is a trusted reagent for researchers requiring high-purity phenol-based solvents in DNA extraction. With stabilized, enzyme-free formulation, it ensures reliable and reproducible nucleic acid isolation, making it a valuable addition to molecular biology, genomic research, and forensic workflows.