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Gibco™ Medium 199, powder, Phenol Red & L-glutamine
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With
• Phenol Red
• L-glutamine
Without
• Sodium Bicarbonate
• HEPES
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Medium 199 was originally formulated for nutritional studies of chick embryo fibroblasts, and has since become a widely adopted basal medium for the cultivation of primary and non-transformed mammalian cells. Its broad applicability and well-balanced composition make it suitable for virology, vaccine production, and explant culture of tissues such as mouse pancreatic epithelium and rat lens.
This powdered version is supplied without sodium bicarbonate and HEPES, and includes L-glutamine, phenol red, and sodium pyruvate. It is designed for reconstitution and requires pH adjustment and sterile filtration before use. Medium 199 uses a sodium bicarbonate buffering system, so it should be prepared and used in a 5–10% CO₂ incubator environment.
Key Features
- With: L-glutamine, Phenol Red, Sodium Pyruvate
- Without: Sodium Bicarbonate, HEPES
- Powder format: Requires reconstitution with sterile water and sodium bicarbonate
- Unique formulation: Contains nucleotides such as adenine, adenosine, thymine, hypoxanthine, and a rich vitamin profile
- Supports a wide range of mammalian cells, especially non-transformed primary cell cultures
- Compatible with CO₂ incubators when supplemented with bicarbonate
- Manufactured under cGMP conditions and ISO 13485 certified
Applications
- Nutritional studies of fibroblasts (e.g., chick embryo fibroblasts)
- Cultivation of non-transformed mammalian cells
- In vitro culture of mouse and rat explants (e.g., pancreatic, lens tissue)
- Research in virology, cytopathology, and vaccine development
- Base medium for custom supplementing in experimental workflows
Specifications
| Property | Details |
|---|---|
| Product Name | Gibco™ Medium 199, Powder |
| Catalog Number | 31100027 / 31100-027 |
| Product Form | Powder |
| Volume After Reconstitution | 1 L |
| Classification | Animal Origin |
| pH Range (before adjustment) | 4.1 to 4.7 |
| Osmolality | 240 – 300 mOsm/kg |
| Buffer System | Requires sodium bicarbonate (not included) |
| With Additives | L-glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | Sodium Bicarbonate, HEPES |
| Culture Type | Mammalian cell culture |
| Culture Environment | CO₂ incubator (5–10%) required after reconstitution |
| Shelf Life | 36 months |
| Storage Conditions | 2–8 °C (refrigerated) |
| Shipping Conditions | Ambient |
| Manufacturing Standards | cGMP-compliant under ISO 13485 |
| Unit Size | 1 L (powder form, pre-weighed for reconstitution) |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Preparation Notes
- Supplement with sodium bicarbonate (e.g., 2.2 g/L) prior to use
- Adjust pH to physiological range (~7.2–7.4) after bicarbonate addition
- Sterile filter after preparation
- Store reconstituted medium at 2–8°C and use within 7–10 days
Gibco™ Medium 199, Powder is a reliable and customizable basal medium for a variety of mammalian cell culture applications. Its rich nutrient profile, flexible format, and broad cell line compatibility make it a valuable tool in virology, vaccine research, and non-transformed cell cultivation. When prepared properly with sodium bicarbonate and filtered, it offers a robust foundation for downstream experimental success.