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Gibco™ DMEM, high glucose, no glutamine, no phenol red, 500 mL
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Cell Culture | ||
Customized Basal Medium for Specialized Mammalian Cell Culture Applications
Gibco™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose, No Glutamine, No Phenol Red is a modified basal medium formulated to support a broad spectrum of mammalian cell types while providing flexibility in supplementation. This DMEM variant is designed for users who prefer to add specific supplements such as glutamine and indicators based on their experimental needs, offering enhanced control over the culture environment.
With high glucose (4.5 g/L) and a simplified formulation (free of L-glutamine, sodium pyruvate, HEPES, and phenol red), this medium is particularly useful in metabolic studies, fluorescent imaging, and glutamine-sensitive assays.
Key Features & Benefits
-
High Glucose (4.5 g/L)
Supports growth and metabolic activity in energy-demanding cells and cell lines. -
No Glutamine
Allows researchers to supplement with their preferred glutamine source (e.g., L-glutamine or GlutaMAX™) based on specific cell line requirements. -
No Phenol Red
Ideal for fluorescence-based assays and hormone-sensitive cell lines, eliminating background interference. -
Free of Sodium Pyruvate and HEPES
Suitable for experiments requiring controlled addition of buffering agents and metabolic substrates. -
Animal-Origin-Free & Sterile-Filtered
Manufactured under cGMP-compliant, ISO 13485-certified conditions for consistency and sterility.
Applications
- Mammalian cell culture with customized supplementation
- Glutamine metabolism studies
- Fluorescent imaging and live-cell assays
- Drug screening and toxicity testing
- Hormone or estrogen receptor-sensitive assays
- Protein expression and transfection protocols
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | DMEM, High Glucose, No Glutamine, No Phenol Red |
| Catalog Number | 31053028 |
| Glucose Concentration | 4500 mg/L (4.5 g/L) |
| Form | Liquid |
| Concentration | 1X |
| With Additives | High Glucose |
| Without Additives | L-Glutamine, Phenol Red, Sodium Pyruvate, HEPES |
| Sterility | Sterile-filtered |
| Buffer System | Sodium bicarbonate (3.7 g/L) |
| CO₂ Requirement | 5–10% CO₂ environment recommended |
| Cell Lines Supported | HeLa, HEK293, Cos-7, PC-12 |
| Primary Cells Supported | Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months from date of manufacture |
| Shipping Conditions | Ambient temperature |
| Manufacturing Quality | cGMP-compliant, ISO 13485 certified |
| Intended Use | For Research Use Only |
Handling Notes
- Supplementation Required: Add L-glutamine or GlutaMAX™ and 5–10% FBS prior to use.
- Use Aseptic Technique: Maintain sterility when handling.
- CO₂ Incubation: Maintain 5–10% CO₂ to stabilize pH via the sodium bicarbonate buffer system.
- Storage: Store at 2–8°C and protect from light.
Gibco™ DMEM, High Glucose, No Glutamine, No Phenol Red offers a highly flexible foundation for mammalian cell culture. Whether your research focuses on metabolic regulation, fluorescent assays, or controlled media supplementation, this medium provides the clean, customizable environment necessary for precision experimentation and consistent cell performance.