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VWR Guanidinium hydrochloride ≥99%, GPR RECTAPUR®, 500g
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CAS Number :
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | VWR | ||
| Product Grade | Molecular Biology | Formula | NH₂C(=NH)NH₂·HCl |
Guanidinium hydrochloride is a high-purity compound widely used in biochemical, molecular biology, and industrial applications. Known for its strong denaturing properties, it is particularly effective in disrupting hydrogen bonds and protein structures, making it indispensable for protein research and purification workflows.
Key Features
- High Purity:
- Minimum assay of 99%, ensuring reliability in sensitive experiments.
- Denaturing Agent:
- Strongly denatures proteins and nucleic acids, facilitating their solubilization and unfolding.
- Versatile Applications:
- Used in protein purification, DNA/RNA isolation, and biochemical research.
Chemical and Physical Properties
| Property | Details |
|---|---|
| Chemical Name | Guanidinium Hydrochloride |
| Synonyms | Guanidine hydrochloride, Aminoformamidine hydrochloride, Guanidine HCl, Guanidinium chloride |
| Molecular Formula | NH₂C(=NH)NH₂·HCl |
| Molecular Weight | 95.53 g/mol |
| CAS Number | 50-01-1 |
| EINECS Number | 200-002-3 |
| MDL Number | MFCD00013026 |
| Melting Point | 180–188 °C |
| Density | 1.354 g/cm³ (at 20 °C) |
| Storage Temperature | Ambient |
| Merck Index Reference | 13,04578 |
Specifications
| Test Parameter | Specification |
|---|---|
| Assay | ≥99% |
| IR Spectrum | Passes test |
| Melting Point | 180–188 °C |
Applications
- Protein Denaturation and Solubilization:
- Efficiently denatures proteins by disrupting hydrogen bonds.
- Commonly used in the preparation of protein solutions for analysis or refolding.
- DNA/RNA Isolation:
- Serves as a chaotropic agent in nucleic acid extraction protocols.
- Helps disrupt cell membranes and denature macromolecules.
- Chromatography:
- Utilized in high-performance liquid chromatography (HPLC) for protein and peptide purification.
- Biochemical Research:
- Essential for studying protein folding and stability.
- Facilitates enzyme inactivation for biochemical assays.
- Industrial Applications:
- Used in the synthesis of pharmaceuticals and agrochemicals.
Advantages
- High Solubility: Easily dissolves in water, ensuring efficient preparation of solutions.
- Consistency: High assay value ensures reproducibility across experiments.
- Versatility: Applicable in a wide range of molecular biology and biochemical workflows.
Storage and Handling
- Storage Conditions: Store at ambient temperature in a dry, well-ventilated area.
- Shelf Life: Stable under recommended storage conditions.
- Packaging: Supplied in moisture-resistant containers to maintain quality.
Safety Information
- Hazard Classification: Irritant; avoid inhalation and skin contact.
- Precautions:
- Wear protective gloves, goggles, and lab coat when handling.
- Work in a well-ventilated area or under a fume hood.
- Disposal: Dispose of according to local environmental regulations for chemical waste.
VWR Chemicals Guanidinium Hydrochloride ≥99%, GPR RECTAPUR® is a premium-grade compound designed for advanced biochemical and molecular biology applications. Its strong denaturing properties, high purity, and broad utility make it a critical reagent in protein and nucleic acid research.