Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), C12
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Specifications:
Application | Protein Electrophoresis & Western Blotting | ||
Storage Temperature | Ambient | ||
Product Type | Buffer | Forms | Powder |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Molecular Biology | ||
The Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), C12 is a highly purified form of SDS, specially designed for protein electrophoresis, solubilization, and other biochemical applications requiring precise solubility parameters. With a purity of over 98% C12 alkyl sulfate, this formulation minimizes contaminants and chain length variability, ensuring reliable results in sensitive protein experiments.
Key Features:
- High Purity:
- Over 98% C12 alkyl sulfate with minimal levels of hexadecyl sulfate (C16 ≤0.8%), which can inhibit protein renaturation.
- Free of secondary chain lengths like C10, C14, and C18, making it ideal for specialized biochemical protocols.
- Popular Anionic Detergent:
- Widely used in protein electrophoresis and protein solubilization methods.
- Essential for cell lysis buffer formulations, including RIPA buffer.
- Specialized Applications:
- Formulated for applications requiring tightly controlled solubility and buffer conditions, such as protein renaturation.
- Easy-to-Use Format:
- Supplied as a white, particulate-free powder for convenient preparation of solutions.
Applications:
- Protein Electrophoresis:
- A key component in SDS-PAGE, facilitating protein denaturation and uniform charge distribution.
- Protein Solubilization:
- Dissolves membrane-bound and insoluble proteins for downstream analysis.
- Cell Lysis:
- Included in lysis buffers like RIPA buffer to disrupt membranes and release cellular contents.
- Biochemical Research:
- Supports experiments requiring precise detergent conditions, such as protein renaturation studies.
Properties:
Property | Details |
---|---|
Molecular Weight | 288.5 g/mol |
Detergent Class | Ionic (Anionic) |
Aggregation Number | 62 |
Micelle Molecular Weight | 18,000 g |
Critical Micelle Concentration (CMC) | 6–8 mM (0.1728–0.2304% w/v) |
Cloud Point | >100°C |
Dialyzable | No |
Specifications:
Feature | Details |
---|---|
Form | White powder |
Solubility | Clear, colorless 10% (aq, w/v) solution |
Purity | C12 ≥98%, C16 ≤0.8% |
Quantity | 500 g |
Storage Conditions | 20°C to 25°C in a dry, cool, ventilated area |
Advantages:
- High Performance:
- Ensures consistency and reproducibility in protein-related experiments.
- Enhanced Purity:
- Reduced contaminants and secondary chain lengths improve experimental reliability.
- Versatile Applications:
- Suitable for a range of biochemical, electrophoretic, and solubilization workflows.
- Stable and Convenient:
- Easy-to-store powder format with a long shelf life under proper conditions.
Storage and Handling:
- Store at 20°C to 25°C in the original container, away from direct sunlight, moisture, and excessive heat.
- Ensure the storage area is dry and well-ventilated.
Related Products:
- Sodium Dodecyl Sulfate (SDS), Lauryl
- Sodium Dodecyl Sulfate (SDS), 20% Solution
Product Details:
Product Name | Catalog Number | Quantity |
---|---|---|
Sodium Dodecyl Sulfate (C12) | 28312 | 500 g |
The Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), C12 is a trusted choice for researchers conducting protein electrophoresis, solubilization, and other precise biochemical applications. Its high purity and specialized formulation ensure consistent and reproducible results in both routine and advanced workflows.