Thermo Scientific™ PageBlue™ Protein Staining Solution, 1L

Thermo Scientific™ PageBlue™ Protein Staining Solution is a sensitive, cost-effective, and easy-to-use colloidal Coomassie G-250-based stain for endpoint visualization of proteins in polyacrylamide gels and on PVDF membranes. Unlike traditional Coomassie R-250 stains, PageBlue™ eliminates the need for methanol and acetic acid, improving laboratory safety while delivering approximately 10× greater sensitivity.

This single-step, ready-to-use staining solution enables rapid protein detection with a linear dynamic range of 5 to 500 ng, making it ideal for visualizing low-abundance proteins with excellent band clarity. It supports in-gel and in-blot (PVDF) applications and can be reused up to three times, increasing cost-efficiency in high-throughput settings.

Key Features

• No methanol or acetic acid: safer for routine lab use
• Ready-to-use format: no dilutions or additions needed
• Fast staining protocol: 25–40 minutes; overnight safe
• High sensitivity: linear range from 5 to 500 ng
• Colorimetric detection: deep blue bands without background interference
• Reusable: up to three times per bottle
• Compatible with SDS-PAGE gels and PVDF membranes

Applications

• Protein visualization after SDS-PAGE
• Endpoint staining in 1D or 2D electrophoresis
• Total protein assessment on PVDF membranes
• General laboratory use in proteomics workflows

Specifications

Conclusion

Thermo Scientific™ PageBlue™ Protein Staining Solution offers a highly sensitive, user-friendly, and economical alternative to traditional Coomassie stains. Its single-step protocol, safety improvements, and strong detection performance make it a preferred choice for routine and advanced protein analysis in electrophoresis applications.