Invitrogen™ Power Blotter Pre-cut Membranes and Filters, PVDF, 0.45 μm, regular size, 13.5 x 8.5 cm, 20 Blots

The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, PVDF, Regular Size are pre-assembled, ready-to-use membrane and filter paper stacks, designed for high-efficiency protein transfer from polyacrylamide gels using the Power Blotter System. These stacks ensure rapid, reproducible, and high-quality Western blot results, allowing protein transfer in less than 10 minutes.

Each stack includes 20 sheets of polyvinylidene difluoride (PVDF) membrane (0.45 μm pore size, low fluorescence) and 80 sheets of absorbent blotting paper (0.85 mm thickness), optimized for semi-dry blotting workflows. These stacks support two mini-size gels or one midi-size gel, making them ideal for standard protein analysis workflows.

Key Features & Benefits

1. Ready-to-Use for Fast & Reliable Protein Transfer

• Pre-cut membranes and filters minimize preparation time and handling errors.
• Designed for regular-size blots (13.5 cm × 8.5 cm), eliminating manual cutting.
• Optimized for Power Blotter 1-Step Transfer Buffer for high-efficiency blotting.

2. High-Performance PVDF Membrane (0.45 μm Pore Size, Low Fluorescence)

• Higher binding capacity than nitrocellulose (240 μg/cm²), enabling sensitive protein detection.
• Pre-activated and ready to use, eliminating the need for alcohol pretreatment.
• Compatible with staining, immunodetection, fluorescence, and radiolabeling detection methods.
• Low background levels and enhanced sensitivity for fluorescent probing compared to standard PVDF or nitrocellulose membranes.

3. Reliable & Reproducible Protein Transfer

• Even membrane thickness and large surface area ensure uniform protein binding.
• Validated for use with the Power Blotter System, ensuring batch-to-batch consistency.
• Reduces transfer variability, providing consistent Western blot performance.

4. Regular-Size Format for Maximum Compatibility

• Dimensions: 13.5 cm × 8.5 cm, suitable for:
  • Two standard mini-size gels (Novex, Bolt, NuPAGE mini gels).
  • One midi-size gel (Invitrogen Novex and NuPAGE midi gels).
• Optimized for high-throughput workflows, reducing processing time and increasing efficiency.

Specifications

Kit Components & Storage

Storage Conditions:

• Store at room temperature in a dry environment.
• Handle membranes with gloves to prevent contamination.
• Keep away from moisture and direct sunlight to maintain optimal performance.

Available Product Variants

Compatible Accessories & Consumables

Applications

1. Western Blotting & Protein Analysis

• Fast and high-efficiency protein transfer for immunodetection and proteomics research.
• Optimized for PVDF-based Western blot detection methods.
• Superior protein retention and signal clarity for fluorescence-based detection.

2. Molecular Biology & Biochemistry Research

• Designed for high-throughput Western blot workflows, improving research efficiency.
• Supports high-sensitivity detection using chemiluminescence, fluorescence, and radiolabeling methods.

3. Biotech & Pharmaceutical Research

• Used for protein expression studies, immunoblotting, and biotherapeutic research.
• High-throughput compatibility with Power Blotter Systems for large-scale experiments.

Storage & Handling

• Store at room temperature in a dry environment.
• Handle with gloves to prevent contamination of PVDF membranes.
• Ensure proper alignment with gels for optimal transfer efficiency.

Ordering Information

The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, PVDF, Regular Size provide fast, efficient, and high-sensitivity semi-dry Western blot protein transfers. Designed for use with Power Blotter Systems, these pre-assembled transfer stacks streamline the blotting process, offering high-protein binding capacity and uniform transfer performance.