Liofilchem™ Trichomonas Broth, 5mL

Liofilchem™ TRICHOMONAS BROTH is a specialized liquid culture medium designed for the selective cultivation, enrichment, and detection of Trichomonas species, particularly Trichomonas vaginalis, from clinical urogenital specimens. This in vitro diagnostic medium provides optimal growth conditions while inhibiting contaminating bacterial flora, ensuring reliable isolation and identification of this clinically significant protozoan pathogen.

Supplied in ready-to-use glass tubes containing 5 mL of medium, TRICHOMONAS BROTH supports the recovery of viable organisms from specimens such as vaginal exudates, urethral swabs, semen, and prostatic fluids. The formulation includes essential nutrients, growth factors, and selective antimicrobial agents to promote protozoan proliferation while maintaining culture integrity.

This medium is widely used in clinical microbiology laboratories, hospital diagnostic facilities, and research institutions for the detection and study of Trichomonas vaginalis, a major causative agent of trichomoniasis, a common sexually transmitted infection (STI).

Key Features

• Selective Cultivation of Trichomonas spp.
  Optimized formulation promotes growth of Trichomonas vaginalis while suppressing bacterial contaminants.
• Ready-to-Use Format
  Supplied in sterile 5 mL glass tubes for immediate inoculation, eliminating preparation time.
• Enriched Nutritional Composition
  Contains tryptone, peptone, yeast extract, maltose, and heat-inactivated horse serum to support optimal protozoan growth.
• Selective Antimicrobial Action
  Chloramphenicol inhibits Gram-positive and Gram-negative bacteria, improving culture selectivity.
• Reducing Environment Support
  L-cysteine and ascorbic acid maintain optimal redox conditions necessary for protozoan survival.
• Reliable Diagnostic Performance
  Validated for recovery of Trichomonas vaginalis from clinical specimens.
• In Vitro Diagnostic Use
  Suitable for professional laboratory diagnostic applications.

Principle of the Method

The medium contains essential nutrients and growth factors necessary for protozoan metabolism and replication:

• Tryptone and Peptone: Provide amino acids, nitrogen, and growth nutrients
• Yeast Extract: Supplies vitamins, particularly B-complex vitamins
• Maltose: Serves as an energy source
• Horse Serum: Provides essential growth factors and lipids
• L-Cysteine and Ascorbic Acid: Maintain a reducing environment favorable for protozoan growth
• Potassium Phosphates: Stabilize pH through buffering action
• Chloramphenicol: Inhibits bacterial contamination

This balanced formulation supports selective proliferation of Trichomonas spp. while preventing interference from competing microorganisms.

Applications

• Clinical microbiology laboratories
• Hospital diagnostic laboratories
• STI diagnostic testing
• Urogenital infection diagnostics
• Protozoan culture and isolation
• Medical research laboratories
• Veterinary and human diagnostic laboratories
• Microbiology teaching laboratories

Specimen Types

Suitable for cultivation of Trichomonas from:

• Vaginal exudates
• Genital secretions
• Urethral specimens
• Semen samples
• Prostatic fluid
• Other urogenital specimens

Technical Specifications

Typical Formula (g/L)

Storage and Stability

• Store at 2–8°C, protected from light
• Transport stable at 18–25°C for up to 4 days
• Transport stable at 35–39°C for up to 48 hours
• Do not use if medium shows contamination or deterioration

Quality Control Performance

Incubation Conditions

• Temperature: 35 ± 2°C
• Incubation time: Up to 3 days
• Examination: Microscopic observation for motile trophozoites

Liofilchem™ TRICHOMONAS BROTH is a reliable, ready-to-use selective culture medium for the isolation and cultivation of Trichomonas vaginalis from clinical specimens. Its enriched formulation, antimicrobial selectivity, and validated performance make it an essential diagnostic tool for clinical microbiology laboratories involved in the detection and study of protozoan infections.