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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Molecular Biology | Formula | HSCH₂CH₂OH |
Gibco™ 2-Mercaptoethanol, also known as β-mercaptoethanol (BME), is a potent thiol-based reducing agent commonly used in mammalian cell culture media to reduce oxidative stress by neutralizing reactive oxygen species (ROS). Supplied as a 55 mM ready-to-use solution in Dulbecco's Phosphate Buffered Saline (DPBS), this formulation ensures convenience, reliability, and compatibility with standard culture protocols.
Due to the instability of 2-mercaptoethanol in aqueous solution, Gibco recommends daily supplementation in long-term cultures. Manufactured under cGMP conditions in an FDA-registered, ISO 13485–certified facility, this product offers the regulatory-grade documentation and consistency required for high-quality research and manufacturing workflows.
Key Features & Benefits
✅ 55 mM Concentration in DPBS – Ready-to-use for mammalian cell culture supplementation
✅ Reduces Oxygen Toxicity – Protects sensitive cells from oxidative damage and free radicals
✅ cGMP-Compliant Manufacturing – Produced in an ISO 13485-certified and FDA-registered facility
✅ Long Shelf Life (36 Months) – Stable when stored at 2°C to 8°C
✅ Ideal for Stem Cells, Primary Cells, and Sensitive Mammalian Cell Lines
✅ Research-Grade Formulation – For use in academic, industrial, and biopharmaceutical R&D
Applications
- Mammalian Cell Culture – Reduces oxidative stress in sensitive cell lines
- Embryonic Stem Cell Maintenance – Supports growth and genomic integrity
- Primary Cell and Hybridoma Cultures – Maintains redox balance in fragile cultures
- Biopharmaceutical and Vaccine Research – Supports consistent culture conditions
- Antioxidant Supplementation in Custom Media – Compatible with most media formulations
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | 2-Mercaptoethanol (BME) |
| Catalog Number | 21985023 |
| Concentration | 55 mM |
| Buffer | Dulbecco's Phosphate Buffered Saline (DPBS) |
| Form | Liquid |
| pH Range | 6.0 – 8.0 |
| Quantity | 50 mL |
| Use Type | For Research Use Only |
| Cell Type Compatibility | Mammalian Cells |
| Manufacturing Standards | cGMP-compliant, ISO 13485 certified |
| Storage Conditions | 2°C to 8°C |
| Shipping Conditions | Room Temperature |
| Shelf Life | 36 months from date of manufacture |
Storage & Handling
- Store at 2°C to 8°C upon receipt
- Use in a chemical fume hood; BME has a strong odor and potential toxicity
- Avoid prolonged exposure to light or air
- Supplement fresh into culture medium daily for optimal results
Gibco™ 2-Mercaptoethanol, 55 mM in DPBS, provides a reliable and high-purity solution for reducing oxidative stress in sensitive mammalian cell cultures. With a long shelf life, cGMP-grade manufacturing, and ready-to-use format, this reagent is an essential supplement for maintaining the viability, growth, and integrity of critical cell lines in both academic research and regulated laboratory environments.