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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit
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Sufficient for 10 labelling reactions, each with 1 to 10 mg of antibody
Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit is a complete reagent system designed for efficient, selective biotin labeling of antibodies, proteins, and cell surface macromolecules. This kit uses the membrane-impermeable Sulfo-NHS-LC-Biotin reagent, which features a medium-length hydrophilic spacer arm for optimal biotin accessibility and minimized steric hindrance. Each kit contains reagents sufficient for 10 labeling reactions, ideal for conjugating 1–10 mg of antibody per reaction.
Key Features and Benefits
Efficient Amine Reactivity
- Contains a Sulfo-NHS ester group that reacts specifically with primary amines (lysine side-chains or N-terminal amines), forming stable, irreversible amide bonds.
Hydrophilic Sulfo Spacer (22.4 Å)
- Medium-length spacer arm ensures excellent balance between labeling efficiency and steric accessibility.
- Water solubility is enhanced through sulfonation, eliminating the need for organic solvents.
Cell-Impermeable
- Ideal for cell surface labeling due to the charged sulfo group, which prevents penetration through intact membranes.
Complete Kit Format
- Includes all necessary reagents, including PBS buffer, desalting columns, avidin, and HABA solution for quantification.
Applications
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Biotinylation of antibodies for:
- ELISA
- Western blotting
- Immunohistochemistry (IHC)
- Flow cytometry
- Surface labeling of live cells and extracellular proteins
- Protein immobilization for affinity purification using streptavidin or avidin systems
- Soluble biotin conjugation without organic solvents
Technical Specifications
| Property | Details |
|---|---|
| Catalog Number | 21435 |
| Labeling Scale | 1 to 10 mg of antibody per reaction |
| Reagent Type | Sulfo-NHS-LC-Biotin |
| Spacer Arm Length | 22.4 Å |
| Solubility | Water |
| Cell Permeability | No (cell-impermeant) |
| Label Type | Biotin |
| Target Functional Group | Primary amines (-NH₂) |
| Labeling Targets | Antibodies, proteins, surface macromolecules |
| Storage Conditions | Biotin and avidin at –20°C; other components at 4°C |
| Shipping Condition | Wet ice |
| Unit Size | 10 reactions |
Kit Contents
- EZ-Link™ Sulfo-NHS-LC-Biotin, 25 mg
- PBS Pack (sufficient to make 500 mL)
- Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL (10 columns)
- HABA Solution, 1 mL
- Avidin, 10 mg
The EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit is an ideal solution for researchers requiring reliable, water-soluble, and membrane-impermeant biotinylation. Its medium-length hydrophilic spacer ensures accessibility for downstream avidin/streptavidin interactions, while the complete kit format simplifies the labeling workflow. Whether for surface protein detection, antibody conjugation, or affinity purification, this reagent system provides high performance and reproducibility in bioconjugation applications.