Thermo Scientific™ EZ-Link™ NHS-Biotin, 100 mg

EZ-Link™ NHS-Biotin is a highly efficient, amine-reactive biotinylation reagent designed for covalent labeling of primary amines on proteins, peptides, antibodies, and other biomolecules. Featuring the classic NHS ester chemistry, it reacts rapidly and irreversibly with the ε-amino groups of lysine residues and the N-terminal amine of polypeptides to form stable amide bonds.

This reagent is membrane-permeable, enabling intracellular labeling, and is ideal for creating biotin-conjugates used in affinity purification, detection, and immobilization assays involving streptavidin, avidin, or NeutrAvidin™.

Key Features & Benefits

• Efficient Amine Reactivity
  Labels lysine residues and N-terminal amines on proteins, peptides, and small molecules.
• Membrane-Permeable
  Suitable for intracellular biotinylation in live-cell or fixed-cell protocols.
• Short Spacer Arm
  Spacer length of ~13.5 Å (native biotin valeric acid) minimizes steric interference and ensures compact conjugates.
• Permanent Labeling
  Forms non-cleavable, irreversible amide bonds, suitable for long-term protein tracking and interaction studies.
• High Purity & Consistency
  Manufactured under rigorous quality standards to ensure reproducibility and optimal conjugation efficiency.
• Organic Solvent Solubility
  Must be initially dissolved in DMSO or DMF prior to dilution into aqueous buffers for reaction.

Applications

• Antibody & Protein Biotinylation
  Label monoclonal or polyclonal antibodies for detection in Western blotting, ELISA, flow cytometry, and IHC.
• Intracellular Labeling
  Introduce biotin inside cells for tracking, proximity labeling, or microscopy-based detection.
• Bioconjugate Synthesis
  Prepare biotinylated peptides or enzymes for downstream affinity capture or protein-protein interaction assays.
• Bead or Surface Conjugation
  Label ligands or receptors for immobilization on streptavidin-coated surfaces or magnetic beads.
• Biotinylation for Mass Spectrometry
  Enable efficient enrichment of biotinylated proteins or peptides prior to LC-MS/MS analysis.

Specifications

Thermo Scientific™ EZ-Link™ NHS-Biotin is a fundamental reagent for robust, high-efficiency biotinylation of amine-containing biomolecules. Its small size, ease of use, and strong biotin-streptavidin binding make it a valuable tool for researchers working in protein labeling, interaction studies, affinity purification, and bioassay development. The membrane-permeable nature further broadens its applications to intracellular targets, offering flexibility and precision in conjugation strategies.