Gibco™ Gentamicin (10 mg/mL)

Gibco™ Gentamicin (10 mg/mL) is a water-soluble antibiotic effective against a broad spectrum of gram-positive and gram-negative bacteria. Derived from the fungus Micromonospora purpurea, gentamicin inhibits protein synthesis by binding to the 30S ribosomal subunit, leading to bacterial death. This product is widely used in cell culture workflows to prevent bacterial contamination.

Key Features:

1. Broad-Spectrum Activity:
   • Effective against gram-positive and gram-negative bacteria, ensuring sterility in various cell culture systems.
2. Sterile-Filtered and Ready-to-Use:
   • Supplied in liquid form, eliminating preparation time and contamination risks.
3. Flexible Working Concentrations:
   • Suitable for use at concentrations ranging from 0.5 to 50 μg/mL, accommodating diverse experimental needs.
4. High Manufacturing Standards:
   • Produced in cGMP-compliant facilities certified to ISO 13485 standards, ensuring consistent quality and reliability.
5. Room Temperature Stability:
   • Stable at 15–30°C, simplifying storage and handling requirements.

Specifications:

Applications:

1. Contamination Prevention:
   • Protects mammalian cell cultures from bacterial contamination.
2. Broad-Spectrum Antimicrobial Activity:
   • Targets a wide variety of bacterial strains in cell culture environments.
3. General Lab Use:
   • Essential for routine laboratory workflows requiring bacterial sterility.
4. Research Applications:
   • Ideal for maintaining sterility during molecular biology, drug screening, and genetic research.

Protocol Overview:

1. Prepare Medium:
   • Dilute Gibco™ Gentamicin to the desired working concentration (0.5–50 μg/mL) in cell culture medium.
2. Sterility Maintenance:
   • Ensure sterility during preparation and handling of the medium.
3. Add to Cell Culture:
   • Incorporate the medium into the culture system and monitor for contamination.

Ordering Information:

Storage and Handling:

• Storage Conditions:
  • Store at 15–30°C to maintain stability.
• Shelf Life:
  • 24 months from the date of manufacture.
• Shipping Conditions:
  • Ships at ambient temperature for convenience.

Advantages:

1. Reliable Contamination Control:
   • Prevents bacterial growth, ensuring reproducibility in experiments.
2. Convenience:
   • Ready-to-use, sterile formulation simplifies experimental workflows.
3. Regulatory Compliance:
   • Manufactured in facilities compliant with cGMP and ISO 13485 standards.
4. Long Shelf Life:
   • Stable for two years, reducing the need for frequent replenishment.
5. Flexible Usage:
   • Compatible with a variety of cell culture types and experimental setups.

Related Products:

• Gibco™ Antibiotic-Antimycotic Solution: Broader antimicrobial coverage.
• Gibco™ Penicillin-Streptomycin Solution: Commonly used dual antibiotic solution.
• Gibco™ DMEM: High-quality medium for mammalian cell culture.

Regulatory Notes:

• For Research Use Only: Not for use in diagnostic or therapeutic applications.

The Gibco™ Gentamicin (10 mg/mL) is a trusted antibiotic solution that delivers reliable protection against bacterial contamination in cell culture workflows. Its high-quality formulation, stability, and flexibility make it an essential reagent for maintaining sterility in research environments.