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Invitrogen™ UltraPure™ Ethidium Bromide, 10 mg/mL, 10 mL
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₂₁H₂₀BrN₃ |
High-Sensitivity Fluorescent Dye for DNA and RNA Detection in Gels
Invitrogen™ UltraPure™ Ethidium Bromide is a ready-to-use aqueous fluorescent dye solution (10 mg/mL) designed for high-sensitivity detection of nucleic acids in agarose gels and CsCl (cesium chloride) gradients. With the ability to detect as little as 1 ng of DNA or RNA, this dye remains a gold standard for nucleic acid visualization in electrophoresis and molecular biology protocols.
The product is formulated using high-purity water and analytical-grade ethidium bromide, ensuring consistent results and low background. Its excitation maxima at 300 nm and 520 nm and emission maximum at 600 nm provide strong fluorescent signals under UV transillumination, making it ideal for post-staining or in-gel detection workflows.
Key Features & Benefits
✅ High Sensitivity – Detects as little as 1 ng of nucleic acid in agarose gels
✅ Versatile Use – Suitable for both DNA and RNA detection
✅ Ready-to-Use 10 mg/mL Solution – No need for dilution unless desired
✅ Compatible with UV Visualization – Strong signal under 300–520 nm excitation
✅ UltraPure™ Quality – Manufactured with low contaminant background for clean gel imaging
✅ Validated for In-Gel and Post-Staining Applications
Applications
- In-Gel DNA or RNA Visualization – After electrophoresis on agarose or acrylamide gels
- Post-Stain Protocols – Enhances visibility of faint bands
- Cesium Chloride Gradient Nucleic Acid Detection
- Routine Molecular Biology and Cloning
- DNA/RNA Fragment Sizing and Quantification
Technical Specifications
| Attribute | Details |
|---|---|
| Product Name | UltraPure™ Ethidium Bromide |
| Catalog Number | 15585011 |
| Form | Liquid |
| Concentration | 10 mg/mL |
| Quantity | 10 mL |
| Label or Dye | Ethidium Bromide |
| Target Molecule | DNA, RNA |
| Detection Method | Fluorescence |
| Detection Location | In-Gel |
| Excitation Maxima | 300 nm and 520 nm |
| Emission Maximum | 600 nm |
| Product Line | UltraPure™ |
| Unit Size | Each |
| Storage Conditions | Store at 15°C to 30°C |
| Shipping Conditions | Ambient |
| Regulatory Use | For Research Use Only – Not for diagnostic use |
Storage & Handling
- Store between 15°C and 30°C in a well-ventilated, light-protected area
- Use gloves and appropriate PPE; ethidium bromide is a known mutagen
- Dispose of according to institutional guidelines for hazardous materials
Invitrogen™ UltraPure™ Ethidium Bromide (10 mg/mL, 10 mL) delivers ultra-sensitive detection of DNA and RNA in gel electrophoresis workflows. Trusted by molecular biology labs worldwide, this product offers the purity, consistency, and performance required for routine and high-sensitivity nucleic acid analysis.