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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Ambient | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-Purity Reagent for Polyacrylamide Gel Electrophoresis (PAGE) of Nucleic Acids and Proteins
Invitrogen™ UltraPure™ Acrylamide is a highly purified reagent, recrystallized three times, and specifically designed for use in polyacrylamide gel electrophoresis (PAGE) for the separation of DNA, RNA, and proteins. This acrylamide powder serves as a key monomer for gel polymerization and supports applications ranging from restriction fragment separation to protein characterization using denaturing (SDS or urea), native, and isoelectric focusing gels.
With consistent performance validated through polymerization time testing in SDS-PAGE systems, UltraPure™ Acrylamide is ideal for high-resolution analytical techniques in molecular biology, biochemistry, and proteomics.
✅ Key Features & Benefits
- Triple-Recrystallized for UltraPurity – Removes impurities and polymerization inhibitors for reliable gel formation
- Versatile Electrophoresis Reagent – Suitable for native, SDS, urea-denaturing, and isoelectric focusing PAGE formats
- High Resolution – Supports precise separation of DNA fragments and proteins
- Validated for SDS-PAGE – Polymerization time tested in standardized gel systems
- Convenient 500 g Format – Ideal for high-volume gel preparation in research labs
🧪 Applications
- DNA and RNA Fragment Analysis – Restriction digests, sequencing (e.g., Sanger, Maxam-Gilbert)
- Protein Electrophoresis – SDS-PAGE, native PAGE, and urea-PAGE
- Isoelectric Focusing – Protein separation based on isoelectric points
- Gel Matrix Preparation – Casting of vertical and horizontal polyacrylamide gels
- Molecular Biology, Genomics, and Proteomics Research
🔧 Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | UltraPure™ Acrylamide |
| Catalog Number | 15512023 |
| Form | Powder |
| Purity | UltraPure™ – triple-recrystallized |
| Quantity | 500 g |
| Use/Application | Gel Electrophoresis |
| Compatibility | PAGE systems for DNA, RNA, and proteins |
| Performance Testing | Polymerization time in SDS-PAGE |
| Storage Conditions | Room Temperature |
| Shipping Conditions | Ambient |
| Regulatory Use | For Research Use Only |
⚠️ Safety & Handling
- Acrylamide is a neurotoxin and suspected carcinogen in monomeric form
- Always wear gloves, goggles, and a lab coat
- Use in a well-ventilated area or fume hood
- Dispose of unpolymerized acrylamide waste according to institutional guidelines
Invitrogen™ UltraPure™ Acrylamide (500 g) is the trusted choice for researchers requiring high-purity monomer for consistent and high-resolution gel electrophoresis. With triple-recrystallization and rigorous performance testing, this reagent ensures clean, reproducible separation of nucleic acids and proteins, making it a cornerstone for accurate analysis in molecular biology and proteomics workflows.