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Gibco™ Trypsin-EDTA (0.5%), no phenol red, 100 mL
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Trypsin-EDTA (0.5%), No Phenol Red is a high-quality, sterile cell dissociation reagent designed for efficient detachment and dissociation of mammalian cells in routine cell culture and tissue processing workflows. Manufactured from irradiated trypsin powder derived from porcine pancreas, this solution provides reliable enzymatic activity for cell passaging, subculturing, and primary tissue dissociation.
This formulation contains EDTA, which enhances dissociation efficiency by chelating calcium and magnesium ions, thereby weakening cell adhesion. The absence of phenol red makes it ideal for applications where indicator dyes may interfere with downstream assays or imaging.
Produced under strict cGMP manufacturing conditions and subjected to rigorous quality testing, Gibco Trypsin-EDTA ensures consistent performance, sterility, and traceability for research and biomanufacturing applications.
Applications
Gibco Trypsin-EDTA (0.5%) is widely used for:
- Mammalian cell culture passaging
- Cell detachment from culture vessels
- Primary tissue dissociation
- Cell harvesting for downstream assays
- Preparation of single-cell suspensions
- Cell line maintenance and expansion
- Biopharmaceutical and biotechnology research
Product Specifications
| Parameter | Specification |
|---|---|
| Product Name | Trypsin-EDTA (0.5%), No Phenol Red |
| Brand | Gibco™ |
| Catalog Number | 15400054 |
| Concentration | 0.5% (10X) |
| Form | Liquid |
| Chelator | EDTA |
| Phenol Red | Not included |
| Cell Type Compatibility | Mammalian cells |
| Osmolality | 280–340 mOsm/kg |
| pH | 7.1–8.0 |
| Sterility | Sterile-filtered |
| Source | Porcine pancreas (animal origin) |
| Product Type | Cell culture dissociation reagent |
| Quality Standard | cGMP manufactured, ISO 13485 certified |
| Shipping Conditions | Wet ice |
| Storage Conditions | −5°C to −20°C |
| Shelf Life | 24 months |
| Application | Research use or further manufacturing |
Quality and Manufacturing
Gibco Trypsin-EDTA is manufactured under strict quality and regulatory standards and undergoes extensive testing for:
- Sterility
- pH and osmolality
- Enzymatic performance
- Endotoxin levels
- Mycoplasma, bacterial, fungal, and viral contamination
- Activity and purity
Each lot includes full documentation such as:
- Certificate of Analysis (COA)
- Animal origin certification
- Irradiation certificate
- Viral inactivation summary
- Complete lot traceability
Ordering Information
| Catalog Number | Product Name | Quantity | Unit |
|---|---|---|---|
| 15400054 | Gibco™ Trypsin-EDTA (0.5%), No Phenol Red | 100 mL | Each |
Storage and Handling
- Store at −5°C to −20°C
- Maintain sterility during use
- Avoid repeated freeze-thaw cycles
- Dilute aseptically with calcium- and magnesium-free buffer if required
Gibco™ Trypsin-EDTA (0.5%), No Phenol Red is a trusted and reliable cell dissociation reagent designed for efficient mammalian cell detachment and culture maintenance. With rigorous quality testing, cGMP manufacturing, and excellent enzymatic performance, it is an essential reagent for cell culture laboratories, biotechnology research, and biopharmaceutical workflows.