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Chromsystems Reagent Kit for HPLC Analysis of Haemoglobin Variants (1000 Tests)
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Kit Content;
15331 - Buffer A, 2100mL x4
15332 - Buffer B, 3000mL x2
15334 - Hemolysis Reagent, 500mL x2
15335 - Wash Buffer, 1000mL x1
15390 - HPLC Column Equilibrated with test chromatogram x1
Specifications:
| Application | chromatography | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Diagnostic kits | Forms | Kit with Various components |
| Product Brand | Chromsystems Instruments & Chemicals GmbH. | ||
| Product Grade | Analytical grade | ||
The Chromsystems Reagent Kit for HPLC Analysis of Hemoglobin Variants is a high-performance diagnostic solution designed for the rapid and accurate separation and quantification of major hemoglobin fractions and variants in whole blood. This kit enables comprehensive profiling of clinically relevant hemoglobins including Hemoglobin A (HbA), Hemoglobin A1c (HbA1c), and variants such as HbS, HbC, HbD, HbE, HbF, and HbA2.
Using advanced HPLC technology, the assay delivers complete separation within just 9 minutes, supporting high-throughput clinical diagnostics and screening programs.
Key Features
- Simultaneous measurement of 8 key hemoglobin variants
- Fast analysis time: 9 minutes per run
- High-resolution separation with clear, identifiable peaks
- Reliable differentiation of HbA2 from HbD and HbE
- Compatible with standard HPLC systems (UV/VIS detection)
- Suitable for high-throughput clinical laboratories (1000 tests/kit)
- Stable and reproducible performance across runs
Measured Parameters
- HbA
- HbA1c
- HbA2
- HbC
- HbD
- HbE
- HbF
- HbS
Clinical Applications
- Screening and diagnosis of hemoglobinopathies
-
Detection of:
- Sickle cell disease (HbS)
- Thalassemias (e.g., β-thalassemia via HbA2 levels)
- Monitoring of glycemic control (HbA1c)
- Evaluation of fetal hemoglobin (HbF) levels
- Routine use in clinical chemistry and hematology laboratories
Analytical Performance
| Parameter | Specification |
|---|---|
| Method | HPLC (UV/VIS detection) |
| Run Time | 9 minutes |
| Injection Volume | 10–20 µL |
| Flow Rate | 1.5 mL/min |
| Wavelength | 415 nm |
| Column Temperature | Ambient (~25°C) |
| Gradient | Binary |
| Max Pressure | 140 bar |
Quantification Limits
| Parameter | Range |
|---|---|
| HbA2 | 2% – ≥75% |
| HbF | 1% – ≥70% |
Precision & Accuracy
- Intra-assay CV: < 8%
- Inter-assay CV: < 4%
-
Recovery:
- HbA2: 100%
- HbF: 99%
Sample Requirements
- Specimen: Whole blood
-
Preparation:
- Dilute 5–8 µL whole blood with 1 mL hemolysis reagent
- Vortex and inject 10–20 µL into HPLC system
Kit Components
| Component | Description |
|---|---|
| Buffer A | Mobile phase |
| Buffer B | Mobile phase |
| Hemolysis Reagent | Sample preparation reagent |
| Wash Buffer | System cleaning solution |
| HPLC Column | Specialized column for hemoglobin separation |
Product Advantages
- Comprehensive profiling of hemoglobin variants in one run
- High throughput suitable for routine diagnostics
- Excellent peak resolution and reproducibility
- Reduces risk of false quantification and misidentification
- Compatible with widely available HPLC systems
This Chromsystems kit provides laboratories with a robust, fast, and accurate solution for hemoglobin variant analysis. Its optimized separation technology ensures clinical reliability, making it ideal for diagnostic laboratories, screening programs, and research institutions.
The Chromsystems HPLC Hemoglobin Variants Kit (1000 tests) is a powerful tool for comprehensive hemoglobin analysis, delivering speed, precision, and reliability in a single workflow. It is well suited for laboratories requiring efficient screening and accurate diagnosis of hemoglobin disorders.