Gibco™ HBSS, no calcium, no magnesium, no phenol red

Gibco™ Hanks' Balanced Salt Solution (HBSS) without calcium, magnesium, and phenol red is a sterile, ready-to-use buffer solution designed for various cell culture applications, including cell washing, transport, and dissociation preparation. This HBSS formulation maintains osmotic balance and pH stability, making it ideal for primary cell isolation, tissue handling, and enzymatic dissociation protocols.

Since this formulation lacks calcium and magnesium, it is particularly suited for cell rinsing before dissociation with chelating agents (e.g., EDTA or trypsin), preventing premature cell aggregation. Additionally, the absence of phenol red minimizes potential interference in sensitive assays and imaging applications.

Key Features & Benefits

1. Optimized for Cell Culture & Tissue Processing
   • Used for cell washing, dilution, and dissociation preparation.
   • Prevents cell clumping in chelation-based dissociation procedures.
2. Calcium & Magnesium-Free for Enzymatic Digestion Compatibility
   • Facilitates trypsin and EDTA-based cell detachment.
   • Reduces potential interference with proteolytic enzymes.
3. Phenol Red-Free for Sensitive Applications
   • Eliminates background signal interference in fluorescence-based assays.
   • Ideal for microscopy and live-cell imaging studies.
4. Sterile & Ready-to-Use, cGMP-Compliant
   • Manufactured under ISO 13485 standards for consistent quality and reproducibility.
   • Pre-filtered and sterile, eliminating contamination risks.
5. Long Shelf Life & Room Temperature Stability
   • Shelf life of 36 months from the date of manufacture.
   • Can be stored at 15°C to 30°C, ensuring ease of use in laboratory settings.

Product Specifications

Use Cases & Applications

1. Cell Washing & Tissue Transport

• Prepares Cells for Downstream Experiments
  • Maintains cell viability and osmotic balance during processing.
  • Suitable for washing cells before media changes or cryopreservation.
• Ideal for Tissue Transport & Storage
  • Prevents osmotic shock and cell stress when transporting tissue samples.
  • Used in biopsies and primary cell research workflows.

2. Enzymatic Cell Dissociation & Trypsinization

• Enhances Trypsin & EDTA Efficiency
  • Removes calcium and magnesium ions to prevent premature cell adhesion.
  • Prepares cells for efficient detachment and dissociation.
• Essential for Stem Cell & Primary Cell Isolation
  • Facilitates gentle dissociation of stem cells and primary tissues.
  • Commonly used in neural, epithelial, and cardiac cell isolations.

3. Serum-Free & Custom Media Preparation

• Versatile for Custom Cell Culture Conditions
  • Works as a base solution for serum-free and chemically defined media.
  • Suitable for nutrient dilution and preparation of assay reagents.
• Reduces Unwanted Interference in Cell-Based Assays
  • Phenol red-free formulation ensures optical clarity in spectrophotometric and fluorescence assays.
  • Used in microscopy and flow cytometry applications.

4. Immunohistochemistry (IHC) & Functional Assays

• Supports Cell Fixation & Staining Protocols
  • Provides buffering conditions suitable for antibody labeling.
  • Used in protein expression and immunohistochemical analysis.
• Maintains Cell Functionality in Physiological Conditions
  • Ensures stable pH balance and electrolyte composition.
  • Ideal for preclinical drug screening and cytotoxicity assays.

Instructions for Use

General Handling & Preparation

1. Pre-Warm Before Use:
   • Warm HBSS to room temperature or 37°C before application.
2. For Cell Washing & Transport:
   • Gently resuspend cells or tissue in HBSS (No Ca²⁺, Mg²⁺).
   • Centrifuge at 300-400 x g for 5 minutes, discard the supernatant, and proceed with culture.
3. For Trypsinization & Dissociation:
   • Wash cells twice with HBSS to remove serum before enzyme treatment.
   • Add trypsin-EDTA solution, incubate for detachment, and neutralize with serum-containing media.
4. For Serum-Free Culture & Assay Preparation:
   • Dilute supplements, growth factors, or media components as required.
   • Use as a base solution for custom formulations.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 15°C to 30°C (Room Temperature).
  • Avoid prolonged light exposure to maintain stability.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Work in a biosafety cabinet (BSC) when handling sterile media.
• Disposal Guidelines:
  • Dispose of used media according to institutional biosafety protocols.
  • Autoclave if required before disposal.

Why Choose Gibco™ HBSS (No Calcium, No Magnesium, No Phenol Red)?

✅ Optimized for cell washing, dissociation, and tissue transport

✅ Prevents premature cell adhesion in enzymatic dissociation protocols

✅ Sterile, ready-to-use formulation ensures reproducible results

✅ Phenol red-free for sensitive applications and imaging assays

✅ Manufactured under cGMP and ISO 13485 quality standards

Gibco™ HBSS (No Calcium, No Magnesium, No Phenol Red) is an essential solution for primary cell isolation, enzymatic digestion, and sensitive cell culture workflows, ensuring high reproducibility and minimal interference in molecular biology and biopharmaceutical applications.