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Gibco™ MEM α, nucleosides, powder, 1L
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With
• Ribonucleosides
• Deoxyribonucleosides
• L-glutamine
• Phenol Red
Without
• Sodium Bicarbonate
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ MEM α (Minimum Essential Medium Alpha) is a widely used basal medium for mammalian cell culture, designed to support the growth of a broad range of adherent and suspension cell lines.
This formulation includes ribonucleosides, deoxyribonucleosides, L-glutamine, and phenol red, making it suitable for routine cell culture as well as selection of DHFR-negative cells in genetic and recombinant expression systems.
Supplied in powder form, this medium allows flexible preparation and customization, enabling laboratories to adjust bicarbonate levels, pH, and supplementation according to specific experimental requirements.
Key Features and Benefits
Optimized for Mammalian Cell Culture
Supports a wide range of cell types, including keratinocytes, primary astrocytes, and melanoma cells.
Enhanced Nutrient Composition
Contains non-essential amino acids, sodium pyruvate, vitamins (B12, biotin, ascorbic acid), and lipoic acid to promote robust cell growth.
Suitable for DHFR Selection Systems
Ideal for use with DHFR-negative cell lines such as DG44 in recombinant protein expression workflows.
Flexible Powder Format
Allows custom preparation, including sodium bicarbonate addition, pH adjustment, and sterile filtration.
Animal Origin-Free Composition
Supports compliance with regulatory and biosafety requirements in research and bioproduction environments.
cGMP Manufacturing Quality
Produced under ISO 13485 standards, ensuring consistent quality and traceability.
Formulation Details
Contains:
- Ribonucleosides
- Deoxyribonucleosides
- L-glutamine
- Phenol red
- Non-essential amino acids
- Sodium pyruvate
- Vitamins (B12, biotin, ascorbic acid)
- Lipoic acid
- Earle’s salts
Does Not Contain:
- Sodium bicarbonate
- HEPES
- Proteins, lipids, or growth factors
Applications
- Mammalian cell culture (adherent and suspension cells)
- Recombinant protein expression
- DHFR-based selection systems (e.g., DG44 cells)
- Cell biology and cancer research
- Tissue culture and primary cell maintenance
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | MEM α (Minimum Essential Medium Alpha) |
| Form | Powder |
| Cell Type | Mammalian cells |
| Culture Environment | 5–10% CO₂ |
| Osmolality | 240–290 mOsm/kg |
| pH (powder) | 3.3–3.9 |
| Additives | Glutamine, Phenol Red, Sodium Pyruvate |
| Excludes | Sodium bicarbonate, HEPES |
| Serum Requirement | Requires supplementation (e.g., 10% FBS) |
| Manufacturing | cGMP, ISO 13485 compliant |
| Classification | Animal origin-free |
Preparation and Handling
- Reconstitute powder in purified water
- Supplement with sodium bicarbonate as required
- Adjust pH to physiological range
- Sterile filter before use
- Use within recommended storage conditions
Storage and Stability
- Store at 2–8°C
- Shelf life: 36 months from date of manufacture
- Ship at ambient temperature
Ordering Information
| Catalog Number | Description | Size |
|---|---|---|
| 11900016 | MEM α, nucleosides, powder | 1 L |
Unique Advantages
- Widely validated medium for diverse mammalian cell lines
- Suitable for both routine culture and genetic selection systems
- Flexible powder format for customized preparation
- High-quality manufacturing standards (cGMP, ISO compliant)
- Reliable performance from Gibco™, a global leader in cell culture
Gibco™ MEM α, Nucleosides, Powder is a versatile and reliable basal medium designed for modern cell culture applications. Its comprehensive nutrient profile, flexibility in preparation, and compatibility with DHFR selection systems make it an essential choice for research, biotechnology, and pharmaceutical laboratories.